Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
Antigen affinity
Immunogène
An amino acid sequence from the N-terminus of human cardiac Troponin C (MDDIYKAAVEQLTEEQKN) was used as the immunogen for this Troponin C antibody (100% homologous in human, mouse and rat).
TNNC1
Reactivité: Humain, Souris, Rat
IF (p), IHC (p)
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
The stated application concentrations are suggested starting amounts. Titration of the Troponin C antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL
Restrictions
For Research Use only
Buffer
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
Stock
-20 °C
Stockage commentaire
After reconstitution, the Troponin C antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Troponin C, also called TNNC1, and TNC, is a protein that in humans is encoded by the TNNC1 gene. Using a monochromosomal hybrid panel, the TNNC1 gene is mapped to chromosome 3, subsequent analysis of the Genebridge 4 radiation hybrid panel localized the gene to 3p in a region consistent with the earlier assignment. Circular dichroism measurements revealed changes in the secondary structures of the TNNC1 mutants A8V, C84Y, and D145E.