Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
Antigen affinity
Immunogène
An amino acid sequence from the C-terminus of human Pleiotrophin (QAESKKKKKEGKKQEKMLD) was used as the immunogen for this Pleiotrophin antibody (100% homologous in human, mouse and rat).
PTN
Reactivité: Humain, Souris, Rat
WB, ELISA
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
The stated application concentrations are suggested starting amounts. Titration of the Pleiotrophin antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL
Restrictions
For Research Use only
Buffer
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
Stock
-20 °C
Stockage commentaire
After reconstitution, the Pleiotrophin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Pleiotrophin(PTN) also known as heparin-binding brain mitogen(HBBM) or heparin-binding growth factor 8(HBGF-8) or neurite growth-promoting factor 1(NEGF1) or heparin affinity regulatory peptide(HARP) or heparin binding growth associated molecule(HB-GAM) is a protein that in humans is encoded by the PTN gene. PTN is the first member of a family of developmentally regulated cytokines. The PTN gene is mapped to 7q33-q34. A mutant PTN that contained only the first N-terminal 40 amino acids was a dominant negative. Pleiotrophin is expressed in the central and peripheral nervous system and also in several non-neural tissues, notably lung, kidney, gut and bone. Pleiotrophin binds to cell-surface nucleolin as a low affinity receptor. This binding can inhibit HIV infection.