CD20 anticorps (AA 1-261)

N° du produit ABIN3042388

Détail du produit anti-CD20 anticorps

Antigène: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Épitope: AA 1-261

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CD20 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for B-lymphocyte antigen CD20(MS4A1) detection. Tested with WB, IHC-P, FCM in Human.

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for B-lymphocyte antigen CD20(MS4A1) detection. Tested with WB, IHC-P, FCM in Human.
Gene Name: membrane-spanning 4-domains, subfamily A, member 1
Protein Name: B-lymphocyte antigen CD20

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human CD20 recombinant protein (Position: M1-D261). Human CD20 shares 75% amino acid (aa) sequence identity with mouse CD20.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for CD20 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Flow Cytometry: Concentration:1-3 μg/1x106 cells, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Références pour anticorps anti-CD20 (ABIN3042388)

Ni, Chen, Zeng, Pei, Du, Tang, Wang, Hu, Zhu, He, Wei, Wang, Ning, Wang, Wang, Ma, Chen, Sun, Tang, Wang, Wang: "In-cell infection: a novel pathway for Epstein-Barr virus infection mediated by cell-in-cell structures." dans: Cell research, Vol. 25, Issue 7, pp. 785-800, (2016) (PubMed).

Yan, Fan, Bai, Hou, Li, Tong: "Zinc Prevents Abdominal Aortic Aneurysm Formation by Induction of A20-Mediated Suppression of NF-κB Pathway." dans: PLoS ONE, Vol. 11, Issue 2, pp. e0148536, (2016) (PubMed).

Détails sur CD20

Antigène: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Autre désignation: MS4A1 (MS4A1 Produits)

Synonymes: anticorps B1, anticorps Bp35, anticorps CD20, anticorps CVID5, anticorps LEU-16, anticorps MS4A2, anticorps S7, anticorps AA960661, anticorps Cd20, anticorps Ly-44, anticorps Ms4a2, anticorps MS4A1, anticorps bp35, anticorps cd20, anticorps ms4a2, anticorps leu-16, anticorps ms4a4c, anticorps cd20-like, anticorps membrane spanning 4-domains A1, anticorps membrane-spanning 4-domains, subfamily A, member 1, anticorps B-lymphocyte antigen CD20, anticorps MS4A1, anticorps Ms4a1, anticorps ms4a1, anticorps LOC101694281

Sujet: CD20, also known as MS4A1, is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. It is mapped to 11q12.2. This gene encodes a member of the membrane-spanning 4A gene family. The function of CD20 is to enable optimal B-cell immune response, specifically against T-independent antigens. It is suspected that CD20 acts as a calcium channel in the cell membrane. What's more, this protein may be involved in the regulation of B-cell activation and proliferation.

Synonyms: APY antibody|ATOPY antibody|B lymphocyte antigen CD20 antibody|B Lymphocyte Cell Surface Antigen B1 antibody|B-lymphocyte antigen CD20 antibody|B-lymphocyte cell-surface antigen B1 antibody|B-lymphocyte surface antigen B1 antibody|B1 antibody|Bp 35 antibody|Bp35 antibody|CD 20 antibody|CD20 antibody|CD20 antigen antibody|CD20 receptor antibody|CD20_HUMAN antibody|Fc epsilon receptor I beta chain antibody|Fc Fragment of IgE high affinity I receptor for beta polypeptide antibody|FCER1B antibody|FCERI antibody|High affinity immunoglobulin epsilon receptor subunit beta antibody|IgE Fc receptor subunit beta antibody|IGEL antibody|IGER antibody|IGHER antibody|LEU16 antibody|LEU16 antibody|leukocyte surface antigen Leu 16 antibody|Leukocyte surface antigen Leu-16 antibody|Ly44 antibody|Membrane spanning 4 domains subfamily A member 2 antibody|Membrane-spanning 4-domains subfamily A member 1 antibody|MS4A1 antibody

ID gène: 931

UniProt: P11836