KIT anticorps (AA 26-285)

N° du produit ABIN3042739

Détail du produit anti-KIT anticorps

Antigène: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Épitope: AA 26-285

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp KIT est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for Mast/stem cell growth factor receptor Kit(KIT) detection. Tested with WB, IHC-P, FCM in Human.

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for Mast/stem cell growth factor receptor Kit(KIT) detection. Tested with WB, IHC-P, FCM in Human.
Gene Name: v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
Protein Name: Mast/stem cell growth factor receptor Kit

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human c-Kit recombinant protein (Position: Q26-S285). Human c-Kit shares 66% amino acid (aa) sequence identity with mouse c-Kit.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for c-Kit is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Flow Cytometry: Concentration:1-3 μg/1x106 cells, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Références pour anticorps anti-KIT (ABIN3042739)

Yang, Zhou, Lan: "Impact of the alterations in the interstitial cells of Cajal on intestinal motility in post-infection irritable bowel syndrome." dans: Molecular medicine reports, Vol. 11, Issue 4, pp. 2735-40, (2015) (PubMed).

Li, Wang, Zhao, Yang, Li, Zhao, Yu, Wang, Meng, Liu, Zhu, Gao, Jia, Yang: "Nuclear factor of activated T cells 5 maintained by Hotair suppression of miR-568 upregulates S100 calcium binding protein A4 to promote breast cancer metastasis." dans: Breast cancer research : BCR, Vol. 16, Issue 5, pp. 454, (2015) (PubMed).

Xu, Ge, Han, Yang, Su, Zhao, Zhao, Yang, Yang: "High-mobility group box 1 expression and lymph node metastasis in intrahepatic cholangiocarcinoma." dans: World journal of gastroenterology : WJG, Vol. 21, Issue 11, pp. 3256-65, (2015) (PubMed).

Jiang, Li, Zhou, Wang, Zhang, Wang: "Colistin-induced apoptosis in PC12 cells: involvement of the mitochondrial apoptotic and death receptor pathways." dans: International journal of molecular medicine, Vol. 33, Issue 5, pp. 1298-304, (2014) (PubMed).

Ban, Fan, Huang, Li, Chen, Zhang, Xu: "Mono-(2-ethylhexyl) phthalate induces injury in human umbilical vein endothelial cells." dans: PLoS ONE, Vol. 9, Issue 5, pp. e97607, (2014) (PubMed).

Tang, Li, Yu, Gao, Liu, Chen, Xing, Liu, Yao: "Quercetin prevents ethanol-induced iron overload by regulating hepcidin through the BMP6/SMAD4 signaling pathway." dans: The Journal of nutritional biochemistry, Vol. 25, Issue 6, pp. 675-82, (2014) (PubMed).

Cheng, Wu, Gao, Xu: "Analysis of influence of baicalin joint resveratrol retention enema on the TNF-?, SIgA, IL-2, IFN-? of rats with respiratory syncytial virus infection." dans: Cell biochemistry and biophysics, Vol. 70, Issue 2, pp. 1305-9, (2014) (PubMed).

Liu, Peng, Lin, Luo, Jiang, Mo, Yung: "Effect of rhynchophylline on the expression of p-CREB and sc-Fos in triatum and hippocampal CA1 area of methamphetamine-induced conditioned place preference rats." dans: Fitoterapia, Vol. 92, pp. 16-22, (2014) (PubMed).

Zhang, Li, Xu, Mei, Zhang, Murong, Sun, Tong: "Changes of c-fos, malondialdehyde and lactate in brain tissue after global cerebral ischemia under different brain temperatures." dans: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, Vol. 34, Issue 3, pp. 354-8, (2014) (PubMed).

Liu, Wang, Du, Liu, Liu, Zhang, Zhang, Hou, Liu, Zhang, Yu: "Wnt1 inhibits hydrogen peroxide-induced apoptosis in mouse cardiac stem cells." dans: PLoS ONE, Vol. 8, Issue 3, pp. e58883, (2013) (PubMed).

Chuangui, Peng, Zhentao: "The expression of high mobility group box 1 is associated with lymph node metastasis and poor prognosis in esophageal squamous cell carcinoma." dans: Pathology oncology research : POR, Vol. 18, Issue 4, pp. 1021-7, (2012) (PubMed).

Bin, Ma, Xu, Shi: "Embryonic hepatocyte transplantation for hepatic cirrhosis: efficacy and mechanism of action." dans: World journal of gastroenterology, Vol. 18, Issue 4, pp. 309-22, (2012) (PubMed).

Lan, Yue, Han, Shi, Yang, Pu, Yao, Kang: "Genome-wide identification of TCF7L2/TCF4 target miRNAs reveals a role for miR-21 in Wnt-driven epithelial cancer." dans: International journal of oncology, Vol. 40, Issue 2, pp. 519-26, (2011) (PubMed).

Bao, Liu, Qin, Xu, Hei, Jaber, Chen: "An immunohistochemical study of S-100 protein in the intestinal tract of Chinese soft-shelled turtle, Pelodiscus sinensis." dans: Research in veterinary science, Vol. 91, Issue 3, pp. e16-24, (2011) (PubMed).

Ge, Zhang, Ye, Tang, Wu: "Ginsenosides promote proliferation of chicken primordial germ cells via PKC-involved activation of NF-kappaB." dans: Cell biology international, Vol. 31, Issue 10, pp. 1251-6, (2007) (PubMed).

Détails sur KIT

Antigène: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Autre désignation: KIT (KIT Produits)

Synonymes: anticorps C-Kit, anticorps CD117, anticorps PBT, anticorps SCFR, anticorps Bs, anticorps Fdc, anticorps Gsfsco1, anticorps Gsfsco5, anticorps Gsfsow3, anticorps SCO1, anticorps SCO5, anticorps SOW3, anticorps Ssm, anticorps Tr-kit, anticorps W, anticorps c-KIT, anticorps c-kit, anticorps MGF, anticorps KIT, anticorps C-KIT, anticorps kit, anticorps receptor, anticorps sparse, anticorps Xkl-1, anticorps kit-a, anticorps kit-b, anticorps kl1-A, anticorps krk1, anticorps pbt, anticorps scfr, anticorps KIT proto-oncogene receptor tyrosine kinase, anticorps v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog a, anticorps KIT proto-oncogene receptor tyrosine kinase S homeolog, anticorps KIT, anticorps Kit, anticorps kita, anticorps kit, anticorps kit.S

Sujet: SCFR(Mast/stem cell growth factor receptor), also known as KIT ONCOGENE or CD117, is a protein that in humans is encoded by the KIT gene. KIT was first described as the cellular homolog of the feline sarcoma viral oncogene v-kit. The KIT gene is mapped on 4q12. Kit was expressed on the surface of germ cells up to the pachytene stage. Signaling from the KIT receptor tyrosine kinase is essential for primordial germ cell growth both in vivo and in vitro. Determination of the KIT effectors acting in primordial germ cells has been hampered by the lack of effective methods to manipulate easily gene expression in these cells.

Synonyms: C Kit antibody|c-Kit antibody|CD 117 antibody|CD117 antibody|CD117 antigen antibody|KIT antibody|KIT oncogene antibody|KIT_HUMAN antibody|Mast cell growth factor receptor antibody|Mast/stem cell growth factor receptor antibody|Mast/stem cell growth factor receptor Kit antibody|p145 c-kit antibody|PBT antibody|Piebald trait protein antibody|Proto oncogene c Kit antibody|Proto oncogene tyrosine protein kinase Kit antibody|Proto-oncogene c-Kit antibody|SCF Receptor antibody|SCFR antibody|soluble KIT variant 1 antibody|Steel Factor Receptor antibody|Stem cell factor receptor antibody|tyrosine protein kinase Kit antibody|Tyrosine-protein kinase Kit antibody|v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog antibody|v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein antibody|v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog antibody

ID gène: 3815

UniProt: P10721

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity