Desmin anticorps (AA 1-304)

N° du produit ABIN3043265

Détail du produit anti-Desmin anticorps

Antigène: Desmin (DES)

Épitope: AA 1-304

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Desmin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Fonction: Rabbit IgG polyclonal antibody for Desmin(DES) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for Desmin(DES) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.
Gene Name: desmin
Protein Name: Desmin

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human Desmin recombinant protein (Position: M1-T304). Human Desmin shares 97% amino acid (aa) sequences identity with both mouse and rat Desmin.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for Desmin is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Références pour anticorps anti-Desmin (ABIN3043265)

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Jiang, Zhang, Fang, Zhang, Lu, Zhu, Cheng, Ai, Zhou, Li, Fang, Yao: "Analgesic effects of Chinese Tuina massage in a rat model of pain." dans: Experimental and therapeutic medicine, Vol. 11, Issue 4, pp. 1367-1374, (2016) (PubMed).

Zhang, Deng, Wen, Chen, Liu, Ye, Huang, Jiang, Xiong: "PPAR? and MyoD are differentially regulated by myostatin in adipose-derived stem cells and muscle satellite cells." dans: Biochemical and biophysical research communications, Vol. 458, Issue 2, pp. 375-80, (2015) (PubMed).

Gao, Zhi, Yang, Li, Yan, Wang: "Low dose of IGF-I increases cell size of skeletal muscle satellite cells via Akt/S6K signaling pathway." dans: Journal of cellular biochemistry, Vol. 116, Issue 11, pp. 2637-48, (2015) (PubMed).

Sondag, Salihoglu, Lababidi, Crowder, Moussa, Abdelmagid, Safadi: "Osteoactivin induces transdifferentiation of C2C12 myoblasts into osteoblasts." dans: Journal of cellular physiology, Vol. 229, Issue 7, pp. 955-66, (2014) (PubMed).

Wei, Yang, Wang, Xu, Liang, Zhou: "Sodium ferulate lowers portal pressure in rats with secondary biliary cirrhosis through the RhoA/Rho-kinase signaling pathway: a preliminary study." dans: International journal of molecular medicine, Vol. 34, Issue 5, pp. 1257-67, (2014) (PubMed).

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Wu, Gu, Zhao, Tan, Chen, Zhu, Zhang: "Derivation of multipotent nestin(+)/CD271 (-)/STRO-1 (-) mesenchymal-like precursors from human embryonic stem cells in chemically defined conditions." dans: Human cell, Vol. 26, Issue 1, pp. 19-27, (2013) (PubMed).

Liu, Liu, Xu, Wang, Zhu, Li, Wang, Liu, Ma, Wu: "Neurotransmitter noradrenaline downregulate cytoskeletal protein expression of VSMCs." dans: Experimental and molecular pathology, Vol. 94, Issue 1, pp. 79-83, (2013) (PubMed).

Yan, Gan, Yang, Sun: "The proliferation and differentiation characteristics of co-cultured porcine preadipocytes and muscle satellite cells in vitro." dans: Molecular biology reports, Vol. 40, Issue 4, pp. 3197-202, (2013) (PubMed).

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Liu, Wang, Yu, Zhang, Chen, Jin, Dai, Li, Xu: "Injection of duck recombinant follistatin fusion protein into duck muscle tissues stimulates satellite cell proliferation and muscle fiber hypertrophy." dans: Applied microbiology and biotechnology, Vol. 94, Issue 5, pp. 1255-63, (2012) (PubMed).

Wang, Yang, Yang, Shu, Wang, Jiang, Yuan, Wu: "The differential proliferative ability of satellite cells in Lantang and Landrace pigs." dans: PLoS ONE, Vol. 7, Issue 3, pp. e32537, (2012) (PubMed).

Zhang, Zheng, Yu, Min, Yu, Ye, Xie, Zhang: "Lentiviral vector-mediated down-regulation of IL-17A receptor in hepatic stellate cells results in decreased secretion of IL-6." dans: World journal of gastroenterology, Vol. 18, Issue 28, pp. 3696-704, (2012) (PubMed).

Longxi, Buwu, Yuan, Sinan: "Expression of p53 in the effects of artesunate on induction of apoptosis and inhibition of proliferation in rat primary hepatic stellate cells." dans: PLoS ONE, Vol. 6, Issue 10, pp. e26500, (2011) (PubMed).

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Xu, Qian, Zhu, Zhang, Yan, Wang, Xu: "Isolation of cancer stem cells from transformed human mesenchymal stem cell line F6." dans: Journal of molecular medicine (Berlin, Germany), Vol. 88, Issue 11, pp. 1181-90, (2010) (PubMed).

Wang, Fan, Zhang, Liu, Sun, Wang: "Compatibility of hyaluronic acid hydrogel and skeletal muscle myoblasts." dans: Biomedical materials (Bristol, England), Vol. 4, Issue 2, pp. 25011, (2009) (PubMed).

Détails sur Desmin

Antigène: Desmin (DES)

Autre désignation: DES (DES Produits)

Synonymes: anticorps DES, anticorps des-b, anticorps MGC80853, anticorps des, anticorps csm1, anticorps csm2, anticorps desm, anticorps cmd1i, anticorps MGC75911, anticorps LOC100220724, anticorps desmin, anticorps cb290, anticorps fb59a12, anticorps wu:fb59a12, anticorps zgc:109859, anticorps CSM1, anticorps CSM2, anticorps LGMD2R, anticorps wu:fc11d08, anticorps zgc:154009, anticorps des-a, anticorps MGC52614, anticorps desmin, anticorps desmin, gene 1 L homeolog, anticorps desmin, gene 1, anticorps desmin a, anticorps desmin b, anticorps desmin, gene 1 S homeolog, anticorps desmin, gene 2 S homeolog, anticorps DES, anticorps des.1.L, anticorps des.1, anticorps des, anticorps desma, anticorps Des, anticorps desmb, anticorps des.1.S, anticorps des.2.S

Sujet: DES, also called desmin, is a protein that in humans is encoded by the DES gene, and this gene is mapped to 2q35. DES is the muscle-specific member of the intermediate filament (IF) protein family. It is one of the earliest myogenic markers, both in heart and somites, and is expressed in satellite stem cells and replicating myoblasts. DES is very important in muscle cell architecture and structure since it connects many components of the cytoplasm. It may be also play an important role in mitochondria function. What's more, DES provides attachments between the terminal Z disc and membrane-associated proteins to form a force-transmitting system that parallels the thin filaments at myotendinous junctions.

Synonyms: CMD1I antibody|CSM1 antibody|CSM2 antibody|DES antibody|DESM_HUMAN antibody|Desmin antibody|FLJ12025 antibody|FLJ39719 antibody|FLJ41013 antibody|FLJ41793 antibody|Intermediate filament protein antibody|OTTHUMP00000064865 antibody

ID gène: 1674

UniProt: P17661