STING/TMEM173 anticorps (C-Term)
-
- Antigène Voir toutes STING/TMEM173 (TMEM173) Anticorps
- STING/TMEM173 (TMEM173) (Transmembrane Protein 173 (TMEM173))
-
Épitope
- AA 284-316, C-Term
-
Reactivité
- Humain
-
Hôte
- Lapin
-
Clonalité
- Polyclonal
-
Conjugué
- Cet anticorp STING/TMEM173 est non-conjugé
-
Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Fonction
- Rabbit IgG polyclonal antibody for Stimulator of interferon genes protein (TMEM173) detection. Tested with WB, IHC-P in Human.
- Séquence
- RLEQAKLFCR TLEDILADAP ESQNNCRLIA YQE
- Réactivité croisée (Details)
- No cross reactivity with other proteins.
- Attributs du produit
-
Rabbit IgG polyclonal antibody for Stimulator of interferon genes protein (TMEM173) detection. Tested with WB, IHC-P in Human.
Gene Name: transmembrane protein 173
Protein Name: Stimulator of interferon genes protein - Purification
- Immunogen affinity purified.
- Immunogène
- A synthetic peptide corresponding to a sequence at the C-terminus of human TMEM173 (284-316aa RLEQAKLFCRTLEDILADAPESQNNCRLIAYQE), different from the related mouse sequence by five amino acids.
- Isotype
- IgG
- Top Product
- Discover our top product TMEM173 Anticorps primaire
-
-
- Indications d'application
-
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. - Commentaires
-
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- Restrictions
- For Research Use only
-
- by
- University of California, Los Angeles
- No.
- #100035
- Date
- 02.07.2016
- Antigène
- Anti-TMEM173 Picoband™ Antibody
- Numéro du lot
- 0951512Da071365
- Application validée
- Immunohistochemistry
- Contrôle positif
- Human pancreatic adenocarcinoma (PDAC)
- Contrôle négative
- Conclusion
- In primary human pancreatic tumor tissue, ABIN3043423 stains specifically the tumor cell cytoplasm only, not fibroblasts.
- Anticorps primaire
- ABIN3043423
- Anticorps secondaire
- Biotin-SP-AffinPure Donkey-anti-rabbit IgG (H+L), Jackson ImmunoResearch, cat#711-065-152
- Full Protocol
- Deparaffinize slides
- Bake slides in oven at 60°C for 1h and let cool completely to RT.
- Rehydrate:
- Xylene 3x 5min
- 100% EtOH 2x 2min
- 95% EtOH 3x 2min
- 70% EtOH 1x 2min
- 50% EtOH 1x 2min
- H2O 2x 3min
- Blocking peroxidase activity
- Treat in 3% H2O2-PBS for 15min on rotator at RT (240 ml/slide hold chamber).
- Wash with PBS 3x 2min.
- Antigen retrieval
- Citrate buffer stock solution 100x, pH6.0 working solution 0.01M, freshly diluted into working solution.
- Boil Citrate buffer until 100°C on hot plate, put slides in the boiled buffer, keep boiling 15min, then let them cool down on bench top for 20min.
- Wash with H2O 2x 2min.
- Wash with PBS 3x 5min.
- PAP-pen cycles the slides: using vacuum to suck off the solution by cycling around the tissue area, then using the PAP-pen draw along the cycle line. Make sure the tissue area is kept wet.
- Apply blocking solution
- Incubate with 50-100µl (cover the whole tissue area) 5% donkey serum in PBS for 1h in moist a box at RT.
- Blocking stock solution: 5% donkey serum in 10 ml PBS.
- Drain blocking solution and blot excess liquid with Kim wipe.
- Prepare primary antibody solution in blocking buffer.
- Apply primary antibody
- Dilute primary TMEM173 antibody ABIN3043423 1:500 dilution in 5% normal goat serum in PBS.
- Incubate overnight in a box at 4°C to assure amoist environment and prevent slides from drying.
- Wash with 0.05% Tween-PBS3x 5min.
- Dilute Biotin-SP-AffinPure Donkey-anti-rabbit IgG (H+L) secondary antibody with 5% blocking solution (5% donkey serum)
- Incubate 1h with secondary antibody at room temperature.
- Prepare ABC solution 1:200: dilute both A and B in 0.05% Tween-PBS. Allow ABC diluted solution to sit for 30-60min before using, keep in the dark.
- Wash with 0.05% Tween-PBS 5min, 7min, and 7min.
- Apply 250µL/slide ABC solution; incubate for 30min at RT in moist incubation box.
- Wash with 0.05% Tween-PBS 5min, 7min, and 7min.
- Filter Hematoxylin.
- Prepare fresh DAB solution in disposable beaker (do not allow solution to sit):
- 2.5ml H2O + 1 drop buffer + 2 drops DAB + 1 drop H2O2
- Use transfer pipette to apply DAB x 1min
- Wash 3x with dH2O (10 dips each).
- Hematoxylin stain 5-10sec.
- Wash until water is clear.
- Hematoxylin stain 5-10sec.
- Dehydrate
- 50% EtOH 1x 2min.
- 70% EtOH 1x 2min.
- 95% EtOH 2x 2min.
- 100% EtOH 2x 2min.
- Xylene 3x 5min.
- Apply cover-slip. Allow glue to dry overnight.
- Notes
Validation #100035 (Immunohistochemistry)Validation ImagesImmunohistochemistry on pancreatic adenocarcinoma (PDAC) FFPE tissue sections. The primary TMEM173 antibody ABIN3043423 was used 1:500 diluted in 5% normal Goat serum in PBS with a biotin-donkey-anti-rabbit IgG (H+L) secondary antibody. A. IHC staining of PDAC tissue from patient #1 at 400x magnification. B. IHC staining of PDAC tissue from patient #2 at 200x magnification.
Protocole -
- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
-
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
-
- Antigène
- STING/TMEM173 (TMEM173) (Transmembrane Protein 173 (TMEM173))
- Autre désignation
- TMEM173 (TMEM173 Produits)
- Synonymes
- anticorps ERIS, anticorps MITA, anticorps MPYS, anticorps NET23, anticorps STING, anticorps 2610307O08Rik, anticorps Mita, anticorps RGD1562552, anticorps transmembrane protein 173, anticorps TMEM173, anticorps Tmem173
- Sujet
-
Transmembrane protein 173 is a protein that in humans is encoded by the TMEM173 gene. This gene encodes a five transmembrane protein that functions as a major regulator of the innate immune response to viral and bacterial infections. The encoded protein is a pattern recognition receptor that detects cytosolic nucleic acids and transmits signals that activate type I interferon responses. Also the encoded protein has been shown to play a role in apoptotic signaling by associating with type II major histocompatibility complex. Mutations in this gene are the cause of infantile-onset STING-associated vasculopathy. Alternate splicing results in multiple transcript variants.
Synonyms: endoplasmic reticulum IFN stimulator antibody|Endoplasmic reticulum interferon stimulator antibody|ERIS antibody|FLJ38577 antibody|hMITA antibody|hSTING antibody|Mediator of IRF3 activation antibody|MITA antibody|Mitochondrial mediator of IRF3 activation antibody|MPYS antibody|N terminal methionine proline tyrosine serine plasma membrane tetraspanner antibody|NET23 antibody|Stimulator of interferon genes antibody|Stimulator of interferon genes protein antibody|STING antibody|TM173_HUMAN antibody|Tmem173 antibody|Transmembrane protein 173 antibody - ID gène
- 340061
- Pathways
- Activation of Innate immune Response
-