CDK2 anticorps (AA 81-298)

N° du produit ABIN3043472

Détail du produit anti-CDK2 anticorps

Antigène: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Épitope: AA 81-298

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CDK2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for Cyclin-dependent kinase 2(CDK2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for Cyclin-dependent kinase 2(CDK2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: cyclin-dependent kinase 2
Protein Name: Cyclin-dependent kinase 2

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human Cdk2 recombinant protein (Position: E81-L298). Human Cdk2 shares 98.6% amino acid (aa) sequence identity with rat Cdk2.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Références pour anticorps anti-CDK2 (ABIN3043472)

Zheng, Yu, Chen, Lu, Fan: "Circulating exosomal microRNAs reveal the mechanism of Fructus Meliae Toosendan-induced liver injury in mice." dans: Scientific reports, Vol. 8, Issue 1, pp. 2832, (2018) (PubMed).

Liu, Lin, Mi, Li, Zhang: "Grape Seed Proanthocyanidin Extract Prevents Ovarian Aging by Inhibiting Oxidative Stress in the Hens." dans: Oxidative medicine and cellular longevity, Vol. 2018, pp. 9390810, (2018) (PubMed).

Ren, Qiu, Lü, Ru, Li, Xiang, Yu, Zhang: "TALENs-directed knockout of the full-length transcription factor Nrf1α that represses malignant behaviour of human hepatocellular carcinoma (HepG2) cells." dans: Scientific reports, Vol. 6, pp. 23775, (2017) (PubMed).

Han, Ge, Zhang, Zhou, Ji, Yang, Zhang: "Effects of tripterygium glycosides on restenosis following endovascular treatment." dans: Molecular medicine reports, Vol. 13, Issue 6, pp. 4959-68, (2017) (PubMed).

Yang, Khan, He, Yao, Li, Gao, Ma: "Tubeimoside-1 induces oxidative stress-mediated apoptosis and G0/G1 phase arrest in human prostate carcinoma cells in vitro." dans: Acta pharmacologica Sinica, Vol. 37, Issue 7, pp. 950-62, (2017) (PubMed).

Zhong, Zhang, Wang, Wang, Song, Zhang, Yu: "Rheum emodin inhibits enterovirus 71 viral replication and affects the host cell cycle environment." dans: Acta pharmacologica Sinica, Vol. 38, Issue 3, pp. 392-401, (2017) (PubMed).

Ren, Huang, Xu, Yang, Yang, Hu: "Isoflavone lupiwighteone induces cytotoxic, apoptotic, and antiangiogenic activities in DU-145 prostate cancer cells." dans: Anti-cancer drugs, Vol. 26, Issue 6, pp. 599-611, (2015) (PubMed).

Zhou, Lu, Liu, Guo, Liu, Zhou, Yang, Mi, Xu: "Platycodin D induces tumor growth arrest by activating FOXO3a expression in prostate cancer in vitro and in vivo." dans: Current cancer drug targets, Vol. 14, Issue 9, pp. 860-71, (2015) (PubMed).

Yu, Zhang, Ren, Zhong, Li, Wang, Li, Liu, Zhao, Zhang, Yu: "Enterovirus 71 mediates cell cycle arrest in S phase through non-structural protein 3D." dans: Cell cycle (Georgetown, Tex.), Vol. 14, Issue 3, pp. 425-36, (2015) (PubMed).

Zhao, Li, Gao, Wang, Yan, Zhan, Liu, Mao, Chen, Wang et al.: "Design, synthesis and biological evaluation of N-alkyl or aryl substituted isoindigo derivatives as potential dual cyclin-dependent kinase 2 (CDK2)/glycogen synthase kinase 3? (GSK-3?) ..." dans: European journal of medicinal chemistry, Vol. 86, pp. 165-74, (2014) (PubMed).

Cheng, Chen, Wang, Ding, Shi: "The effects of urokinase-type plasminogen activator (uPA) on cell proliferation and phenotypic transformation of rat mesangial cells induced by high glucose." dans: Diabetes research and clinical practice, Vol. 103, Issue 3, pp. 489-95, (2014) (PubMed).

Ye, Chen, Chen, Xie, Yang, Lou, Yu: "XJW20, a novel oxoindole derivative, induces G2/M arrest and apoptosis selectively in K562 leukemia cell line." dans: Chemico-biological interactions, Vol. 183, Issue 1, pp. 133-41, (2009) (PubMed).

Détails sur CDK2

Antigène: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Autre désignation: CDK2 (CDK2 Produits)

Synonymes: anticorps p33(CDK2), anticorps A630093N05Rik, anticorps wu:fa10c02, anticorps zgc:56598, anticorps zgc:77684, anticorps eg1, anticorps CDKN2, anticorps cdk2L, anticorps cyclin dependent kinase 2, anticorps cyclin-dependent kinase 2, anticorps cyclin-dependent kinase 2 S homeolog, anticorps CDK2, anticorps Cdk2, anticorps cdk2, anticorps cdk2.S

Sujet: CDK2, Cyclin-Dependent Kinase2, is also known as P33. The CDK2 protein was highly homologous to p34(CDC2) kinase and more significantly homologous to Xenopus Eg1 kinase, suggesting that CDK2 is the human homolog of Eg1. The CDK2 gene is mapped to 12q13, the same region to which the CDK4 gene maps. Human cyclin A binds independently to 2 kinases, p34(cdc2) or p33. In adenovirus-transformed cells, the viral E1A oncoprotein seems to associate with p33/cyclin A but not with p34(cdc2)/cyclin A. The gene for p33 shares 65 % sequence identity with p34(cdc2). P33(cdk2) plays a unique role in cell cycle regulation of vertebrate cells.

Synonyms: Cdc2 related protein kinase antibody|cdc2-related protein kinase antibody|Cdk 2 antibody| CDK2 antibody|CDK2_HUMAN antibody|Cell devision kinase 2 antibody|Cell division kinase 2 antibody|Cell division protein kinase 2 antibody|Cyclin dependent kinase 2 antibody|cyclin dependent kinase 2-alpha antibody|Cyclin-dependent kinase 2 antibody|p33 protein kinase antibody|p33(CDK2) antibody

ID gène: 1017

UniProt: P24941

Pathways: Signalisation PI3K-Akt, Cycle Cellulaire, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA