Annexin V anticorps (AA 2-320)

N° du produit ABIN3043502

Détail du produit anti-Annexin V anticorps

Antigène: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Épitope: AA 2-320

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Annexin V est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Fonction: Rabbit IgG polyclonal antibody for Annexin A5(ANXA5) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for Annexin A5(ANXA5) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.
Gene Name: annexin A5
Protein Name: Annexin A5

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human Annexin V recombinant protein (Position: A2-D320). Human Annexin V shares 94% and 92% amino acid (aa) sequences identity with mouse and rat Annexin V, respectively.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, The detection limit for Annexin V is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Références pour anticorps anti-Annexin V (ABIN3043502)

Manville, Abbott: "Ancient and modern anticonvulsants act synergistically in a KCNQ potassium channel binding pocket." dans: Nature communications, Vol. 9, Issue 1, pp. 3845, (2018) (PubMed).

Sun, Kang, Yao, Chen, Sun, An, Jiang, Wang, Hu: "Dehydrocostus lactone suppressed the proliferation, migration, and invasion of colorectal carcinoma through the downregulation of eIF4E expression." dans: Anti-cancer drugs, Vol. 26, Issue 6, pp. 641-8, (2015) (PubMed).

Ji, Li, Wei, Kong, Jin, Chen, Li, Deng: "Effect of celastrol on growth inhibition of prostate cancer cells through the regulation of hERG channel in vitro." dans: BioMed research international, Vol. 2015, pp. 308475, (2015) (PubMed).

Xu, Bi, Li, Fang, Zhou, Li, Chi, Wan, Shen: "Morphological and proteomic analyses reveal that unsaturated guluronate oligosaccharide modulates multiple functional pathways in murine macrophage RAW264.7 cells." dans: Marine drugs, Vol. 13, Issue 4, pp. 1798-818, (2015) (PubMed).

Hu, Dong, Shi, Deng, Li, Lu: "Evaluation of a novel tetra-functional branched poly(ethylene glycol) crosslinker for manufacture of crosslinked, decellularized, porcine aortic valve leaflets." dans: Journal of biomedical materials research. Part B, Applied biomaterials, Vol. 102, Issue 2, pp. 322-36, (2014) (PubMed).

Xiong, Ma, Zhang, Shao, Zhang, Zhou: "Apoptosis induced by ?,?-dimethylacrylshikonin is associated with Bcl-2 and NF-?B in human breast carcinoma MCF-7 cells." dans: Oncology letters, Vol. 6, Issue 6, pp. 1789-1793, (2013) (PubMed).

Jin, Liu, Wang, Xu, Yang, Lu, Sun, Hu: "Protective effects of [Gly14]-Humanin on beta-amyloid-induced PC12 cell death by preventing mitochondrial dysfunction." dans: Neurochemistry international, Vol. 56, Issue 3, pp. 417-23, (2010) (PubMed).

Détails sur Annexin V

Antigène: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Autre désignation: ANXA5 (ANXA5 Produits)

Synonymes: anticorps anx, anticorps anx5, anticorps ANX V, anticorps anxa5, anticorps cb989, anticorps wu:fa98f06, anticorps wu:fj10f10, anticorps MGC89158, anticorps ANX5, anticorps ENX2, anticorps PP4, anticorps RPRGL3, anticorps Anx5, anticorps R74653, anticorps LC5, anticorps enx2, anticorps annexin A5, anticorps annexin A5b, anticorps Annexin A5, anticorps annexin A5 L homeolog, anticorps ANXA5, anticorps anxa5b, anticorps anxa5, anticorps Anxa5, anticorps anxa5.L

Sujet: Annexin A5 (or annexin V) is a cellular protein in the annexin group. It is mapped to 4q27. Annexin A5 has been proposed to play a role in the inhibition of blood coagulation by competing for phosphatidylserine binding sites with prothrombin and also to inhibit the activity of phospholipase A1. This protein is an anticoagulant protein, it can form a shield around negatively-charged phospholipid molecules, the formation of an annexin A5 shield blocks the entry of phospholipids into coagulation (clotting) reactions. And Annexin A5 can also act as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.

Synonyms: Anchorin CII antibody|Annexin 5 antibody|Annexin A5 antibody|Annexin V antibody|Annexin-5 antibody|Annexin5 antibody|AnnexinA5 antibody|AnnexinV antibody|ANX 5 antibody|ANX A5 antibody|ANX5 antibody|ANXA5 antibody|ANXA5_HUMAN antibody|Calphobindin I antibody|CBP I antibody|CBP-I antibody|Endonexin II antibody|ENX 2 antibody|ENX2 antibody|Lipocortin V antibody|PAP I antibody|PAP-I antibody|Placental anticoagulant protein 4 antibody|Placental anticoagulant protein I antibody|PLACENTAL PROTEIN 4 antibody|PP 4 antibody|PP4 antibody|Thromboplastin inhibitor antibody|VAC alpha antibody|VAC-alpha antibody|Vascular anticoagulant alpha antibody|Vascular anticoagulant-alpha antibody

ID gène: 308

UniProt: P08758

Pathways: Apoptose