CD31 anticorps (AA 28-382)

N° du produit ABIN3043601

Détail du produit anti-CD31 anticorps

Antigène: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Épitope: AA 28-382

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CD31 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for Platelet endothelial cell adhesion molecule(PECAM1) detection. Tested with WB, IHC-P, FCM in Human.

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for Platelet endothelial cell adhesion molecule(PECAM1) detection. Tested with WB, IHC-P, FCM in Human.
Gene Name: platelet/endothelial cell adhesion molecule 1
Protein Name: Platelet endothelial cell adhesion molecule

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human CD31 recombinant protein (Position: Q28-G382). Human CD31 shares 65% and 68% amino acid (aa) sequences identity with mouse and rat CD31, respectively.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for CD31 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Flow Cytometry: Concentration:1-3 μg/1x106 cells, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Références pour anticorps anti-CD31 (ABIN3043601)

Zhang, Chen, Wang, Yang, Li, Wang, Liu, Ye: "Treatment of diabetes mellitus-induced erectile dysfunction using endothelial progenitor cells genetically modified with human telomerase reverse transcriptase." dans: Oncotarget, Vol. 7, Issue 26, pp. 39302-39315, (2018) (PubMed).

Chen, Li, Peng, Jie, Dongye, Cai, Feng, Li, Zeng, Lun, Chen, Xu: "The induction of autophagy against mitochondria-mediated apoptosis in lung cancer cells by a ruthenium (II) imidazole complex." dans: Oncotarget, Vol. 7, Issue 49, pp. 80716-80734, (2018) (PubMed).

Zhang, Peng, Yuan, Xie, Yang, Xue: "Peroxisome proliferator‑activated receptor γ mediates porcine placental angiogenesis through hypoxia inducible factor‑, vascular endothelial growth factor‑ and angiopoietin‑mediated signaling." dans: Molecular medicine reports, Vol. 16, Issue 3, pp. 2636-2644, (2018) (PubMed).

Fan, Qu, Chu, Gao, Gao, Chen, Tian: "MicroRNA-210 promotes angiogenesis in acute myocardial infarction." dans: Molecular medicine reports, Vol. 17, Issue 4, pp. 5658-5665, (2018) (PubMed).

Atala: "Re: Treatment of Diabetes Mellitus-Induced Erectile Dysfunction Using Endothelial Progenitor Cells Genetically Modified with Human Telomerase Reverse Transcriptase." dans: The Journal of urology, Vol. 197, Issue 2, pp. 494, (2017) (PubMed).

Zhou, He, Liu, Feng, Zhang, Hua, Tang: "Angiogenic gene-modified myoblasts promote vascularization during repair of skeletal muscle defects." dans: Journal of tissue engineering and regenerative medicine, Vol. 9, Issue 12, pp. 1404-16, (2016) (PubMed).

Chen, Yu, Gumireddy, Li, Yao, Gao, Chen, Hao, Wang, Huang, Xu, Ye: "ZBRK1, a novel tumor suppressor, activates VHL gene transcription through formation of a complex with VHL and p300 in renal cancer." dans: Oncotarget, Vol. 6, Issue 9, pp. 6959-76, (2016) (PubMed).

Wang, Yang, Tang, Long, Huang: "Wound Dressing Model of Human Umbilical Cord Mesenchymal Stem Cells-Alginates Complex Promotes Skin Wound Healing by Paracrine Signaling." dans: Stem cells international, Vol. 2016, pp. 3269267, (2016) (PubMed).

Zhang, Wang, Yang, Pan, Li, Yuan: "Preclinical pharmacodynamic evaluation of antibiotic nitroxoline for anticancer drug repurposing." dans: Oncology letters, Vol. 11, Issue 5, pp. 3265-3272, (2016) (PubMed).

Zeng, Zhao, Ouyang, Zhao, Zhang, Chen, Yu, Lei: "Label-retaining assay enriches tumor-initiating cells in glioblastoma spheres cultivated in serum-free medium." dans: Oncology letters, Vol. 12, Issue 2, pp. 815-824, (2016) (PubMed).

Li, Zeng, Qi, Tang, Zhang, Wu, Liang, Shi, Liu, Zhang: "Xenotransplantation of human adipose-derived stem cells in zebrafish embryos." dans: PLoS ONE, Vol. 10, Issue 4, pp. e0123264, (2015) (PubMed).

Wang, Yin, Dong, Zhao, Wang, Wang, Wang, Du, Lu: "Inhibition of Janus kinase-2 signalling pathway ameliorates portal hypertensive syndrome in partial portal hypertensive and liver cirrhosis rats." dans: Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver, Vol. 47, Issue 4, pp. 315-23, (2015) (PubMed).

Xu, Wang: "Fluid and cell behaviors along a 3D printed alginate/gelatin/fibrin channel." dans: Biotechnology and bioengineering, Vol. 112, Issue 8, pp. 1683-95, (2015) (PubMed).

Xiao, Qiu, Cui, Ni, Li, Liao, Lu, Ding: "MicroRNA-885-3p inhibits the growth of HT-29 colon cancer cell xenografts by disrupting angiogenesis via targeting BMPR1A and blocking BMP/Smad/Id1 signaling." dans: Oncogene, Vol. 34, Issue 15, pp. 1968-78, (2015) (PubMed).

Huan, Wang, Yang, Cui, Wen, Chen, Chen: "Expression and function of the ID1 gene during transforming growth factor-?1-induced differentiation of human embryonic stem cells to endothelial cells." dans: Cellular reprogramming, Vol. 17, Issue 1, pp. 59-68, (2015) (PubMed).

Liu, Wang, Ye, Yin, Chen: "Distinct molecular basis for endothelial differentiation: gene expression profiles of human mesenchymal stem cells versus umbilical vein endothelial cells." dans: Cellular immunology, Vol. 289, Issue 1-2, pp. 7-14, (2014) (PubMed).

Lu, Liu, Zhu, Xu: "Expression of nerve growth factor and hypoxia inducible factor-1? and its correlation with angiogenesis in non-small cell lung cancer." dans: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, Vol. 34, Issue 3, pp. 359-62, (2014) (PubMed).

Wang, Jiang, Wang, Li, Zhang, Zhao, Peng, Cao, Sun: "Construction of tissue-engineered bone using a bioreactor and platelet-rich plasma." dans: Experimental and therapeutic medicine, Vol. 8, Issue 2, pp. 413-418, (2014) (PubMed).

Wang, Yang, Wen, Ma, Wang, Yu, Tang, Wu, Dong, Cheng, Nie, Chen: "SKLB-M8 induces apoptosis through the AKT/mTOR signaling pathway in melanoma models and inhibits angiogenesis with decrease of ERK1/2 phosphorylation." dans: Journal of pharmacological sciences, Vol. 126, Issue 3, pp. 198-207, (2014) (PubMed).

Xiu, Fan, Li, Cui, Lei: "Different angiogenic abilities of self-setting calcium phosphate cement scaffolds consisting of different proportions of fibrin glue." dans: BioMed research international, Vol. 2014, pp. 785146, (2014) (PubMed).

Détails sur CD31

Antigène: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Autre désignation: PECAM1 (PECAM1 Produits)

Synonymes: anticorps CD31, anticorps CD31/EndoCAM, anticorps GPIIA', anticorps PECA1, anticorps PECAM-1, anticorps endoCAM, anticorps C85791, anticorps Cd31, anticorps Pecam, anticorps platelet/endothelial cell adhesion molecule 1, anticorps platelet and endothelial cell adhesion molecule 1, anticorps pecam1, anticorps PECAM1, anticorps Pecam1

Sujet: CD31 also known as Platelet endothelial cell adhesion molecule (PECAM-1), is a protein that in human is encoded by the PECAM1 gene. Encoded protein is a member of the immunoglobulin superfamily, CD31 is mapped to 17q23.3. CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. It is demonstrated that CD31 expression on human PBSCs may positively affect both neutrophil and platelet engraftment. Meanwhile, CD31 is involved in leukocyte migration and angiogenesis, which are key components of venous thrombus resolution.

Synonyms: Adhesion molecule antibody|CD31 antibody|CD31 antigen antibody|CD31 EndoCAM antibody|Endocam antibody|FLJ34100 antibody|FLJ58394 antibody|GPIIA antibody|GPIIA' antibody|PECA1 antibody|PECA1_HUMAN antibody|Pecam 1 antibody|PECAM 1 CD31 EndoCAM antibody|PECAM antibody|PECAM-1 antibody|Pecam1 antibody|Platelet endothelial cell adhesion molecule antibody|Platelet/endothelial cell adhesion molecule 1 antibody|Platelet/endothelial cell adhesion molecule antibody

ID gène: 5175

UniProt: P16284

Pathways: Regulation of Actin Filament Polymerization