APEX1 anticorps (AA 2-318)

N° du produit ABIN3043785

Détail du produit anti-APEX1 anticorps

Antigène: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Épitope: AA 2-318

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp APEX1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for DNA-(apurinic or apyrimidinic site) lyase(APEX1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for DNA-(apurinic or apyrimidinic site) lyase(APEX1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: APEX nuclease (multifunctional DNA repair enzyme) 1
Protein Name: DNA-(apurinic or apyrimidinic site) lyase

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human APE1 recombinant protein (Position: P2-L318). Human APE1 shares 94% and 93% amino acid (aa) sequences identity with mouse and rat APE1, respectively.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for APE1 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Détails sur APEX1

Antigène: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Autre désignation: APEX1 (APEX1 Produits)

Synonymes: anticorps apex1, anticorps MGC76280, anticorps MGC79645, anticorps MGC78928, anticorps APE, anticorps APE1, anticorps APEN, anticorps APEX, anticorps APX, anticorps HAP1, anticorps REF1, anticorps Apex, anticorps REF-1, anticorps BAP1, anticorps apex1a, anticorps hm:zehn1532, anticorps wu:fc13d11, anticorps zgc:66204, anticorps Ref-1, anticorps apurinic/apyrimidinic endodeoxyribonuclease 1, anticorps APEX nuclease (multifunctional DNA repair enzyme) 1, anticorps APEX nuclease (multifunctional DNA repair enzyme) 1 L homeolog, anticorps DNA-(apurinic or apyrimidinic site) lyase, anticorps apurinic/apyrimidinic endonuclease 1, anticorps APEX1, anticorps apex1, anticorps apex1.L, anticorps CNG03640, anticorps CNH01280, anticorps nth, anticorps Spirs_4119, anticorps Palpr_0103, anticorps Apex1

Sujet: APEX1, also called apurinic endonuclease (APE), is a DNA repair enzyme having apurinic/apyrimidinic (AP) endonuclease, 3-prime, 5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. The human APEX1 gene consists of 5 exons spanning 2.64 kb and exists as a single copy in the haploid genome. Using in situ hybridization, the APEX1 gene is mapped to 14q11.2-q12. The predicted APEX1 protein, which contained probable nuclear transport signals, was identified as a member of a family of DNA repair enzymes found in lower organisms. The abundance of the large form of APEX1 was increased in leiomyoma extracts relative to myometrial tissue extracts, and the large form was dominant in cell lines derived from leiomyosarcomas. The exonuclease activity of nuclear APEX1 can remove the anti-HIV nucleoside analogs AZT and D4T from the 3-prime terminus of a nick more efficiently than can cytosolic exonucleases.

Synonyms: AP endonuclease 1 antibody|AP endonuclease class I antibody|AP lyase antibody|APE 1 antibody|APE antibody|APE-1 antibody|APEN antibody|APEX 1 antibody|APEX antibody|APEX nuclease (multifunctional DNA repair enzyme) 1 antibody|Apex nuclease 1 antibody|Apex nuclease antibody|APEX1 antibody|APEX1_HUMAN antibody|Apurinic endonuclease antibody|Apurinic-apyrimidinic endonuclease 1 antibody|Apurinic/apyrimidinic (abasic) endonuclease antibody|Apurinic/apyrimidinic endonuclease 1 antibody|Apurinic/apyrimidinic exonuclease antibody|APX antibody|BAP1 antibody|Deoxyribonuclease (apurinic or apyrimidinic) antibody|DNA (apurinic or apyrimidinic site) lyase antibody|DNA-(apurinic or apyrimidinic site) lyase, mitochondrial antibody|EC 4.2.99.18 antibody|HAP 1 antibody|HAP1 antibody|Human Apurinic endonuclease 1 antibody|MGC139790 antibody|Multifunctional DNA repair enzyme antibody|Redox factor 1 antibody|Redox factor-1 antibody|REF 1 antibody|REF 1 protein antibody|REF-1 antibody|REF1 antibody|REF1 protein antibody

ID gène: 328

UniProt: P27695

Pathways: Réparation de l'ADN, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus