MMP7 anticorps (AA 1-267)

N° du produit ABIN3043880

Détail du produit anti-MMP7 anticorps

Antigène: MMP7 (Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7))

Épitope: AA 1-267

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MMP7 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for Matrilysin(MMP7) detection. Tested with WB, IHC-P, ELISA in Human.

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for Matrilysin(MMP7) detection. Tested with WB, IHC-P, ELISA in Human.
Gene Name: matrix metallopeptidase 7 (matrilysin, uterine)
Protein Name: Matrilysin

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human MMP7 recombinant protein (Position: M1-K267). Human MMP7 shares 71% amino acid (aa) sequences identity with both mouse and rat MMP7.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for MMP7 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Human

Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Références pour anticorps anti-MMP7 (ABIN3043880)

Li, Xiao, Li, Li, Zeng, Liu, Liang, Li, Chu, Yang: "Hydrogen sulfide reduced renal tissue fibrosis by regulating autophagy in diabetic rats." dans: Molecular medicine reports, Vol. 16, Issue 2, pp. 1715-1722, (2018) (PubMed).

Jia, Jiang, Liu, Wang, Zhu, Zhu, Liu, Zhong, Xie, Huang, Jia, Li, Liu, Zuo, Cheng, Dai, Ren: "Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells." dans: International journal of oncology, Vol. 52, Issue 6, pp. 1787-1800, (2018) (PubMed).

Bai, Luo: "5-Hydroxy-4'-Nitro-7-Propionyloxy-Genistein Inhibited Invasion and Metastasis via Inactivating Wnt/b-Catenin Signal Pathway in Human Endometrial Carcinoma Ji Endometrial Cells." dans: Medical science monitor : international medical journal of experimental and clinical research, Vol. 24, pp. 3230-3243, (2018) (PubMed).

Xu, Xu, Li, Wang, Fu, Jia, Wang, Zhu, Lu, Yao: "Growth differentiation factor 15 induces growth and metastasis of human liver cancer stem-like cells via AKT/GSK-3β/β-catenin signaling." dans: Oncotarget, Vol. 8, Issue 10, pp. 16972-16987, (2017) (PubMed).

Gao, Tang, He, Liu, Mao, Ji, Lin, Wu: "Glycyrrhizic acid alleviates bleomycin-induced pulmonary fibrosis in rats." dans: Frontiers in pharmacology, Vol. 6, pp. 215, (2015) (PubMed).

Yang, Gao, Rao, Zhang, Ouyang, Yang: "Antisense oligonucleotide targeting matrix metalloproteinase-7 (MMP-7) changes the ultrastructure of human A549 lung adenocarcinoma cells." dans: Ultrastructural pathology, Vol. 35, Issue 6, pp. 256-9, (2011) (PubMed).

Yang, Shi, Li, Yi: "Effects of shRNA targeting maspin on invasion of gastric carcinoma SGC7901 cell line." dans: Oncology reports, Vol. 25, Issue 1, pp. 259-65, (2010) (PubMed).

Pan, Zhang, Xu, Meng, Zhang, Xue, Han, Li: "Beta-catenin signaling involves HGF-enhanced HepG2 scattering through activating MMP-7 transcription." dans: Histochemistry and cell biology, Vol. 134, Issue 3, pp. 285-95, (2010) (PubMed).

Détails sur MMP7

Antigène: MMP7 (Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7))

Autre désignation: MMP7 (MMP7 Produits)

Synonymes: anticorps MAT, anticorps MPMM, anticorps MMP-7, anticorps MPSL1, anticorps PUMP-1, anticorps matrilysin, anticorps LOC727698, anticorps MMP7, anticorps Matrilysin, anticorps matrix metallopeptidase 7, anticorps MMP7, anticorps Mmp7

Sujet: Matrix metalloproteinase-7 (MMP-7) previously called putative metalloproteinase I (PUMP1) or matrilysin. The MMP-7 gene has been identified through studies of collagenase-related connective-tissue-degrading metalloproteinases produced by human tumors. The MMP-7 protein has 267 amino acids and is significantly shorter than stromelysin or collagenase (477 and 469 amino acids, respectively). Matrix metalloproteinases play a crucial role in tumor invasion and metastasis. MMP-7, a member of the matrix metalloproteinase family, is structurally different from the other matrix metalloproteinases by virtue of the absence of a conserved COOH-terminal protein domain. In addition, MMP-7 mRNA is regulated in a specific and distinct manner in normal and malignant tissues. MMP-7 has been shown to correlate with nodal or distant metastasis in colorectal carcinomas, however, its implication in early invasive colorectal carcinomas has not been determined. MMP-7 is also a mediator of pulmonary fibrosis and a potential therapeutic target. The standard product used in this kit is recombinant human MMP-7, consisting of 250 amino acids with the molecular mass of 28KDa. The detected MMP-7 includes zymogen and active enzyme.

Synonyms: Matrilysin antibody|Matrilysin uterine antibody|Matrin antibody|Matrix Metalloproteinase 7 antibody|Matrix metalloproteinase-7 antibody|MMP 7 antibody|MMP-7 antibody|MMP7 antibody|MMP7_HUMAN antibody|MPSL1 antibody|PUMP 1 antibody|Pump 1 protease antibody|Pump-1 protease antibody|PUMP1 antibody|Uterine Matrilysin antibody|Uterine metalloproteinase antibody

ID gène: 4316

UniProt: P09237

Pathways: Production of Molecular Mediator of Immune Response