MSH2 anticorps (AA 337-583)

N° du produit ABIN3043885

Détail du produit anti-MSH2 anticorps

Antigène: MSH2 (Mismatch Repair Protein 2 (MSH2))

Épitope: AA 337-583

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MSH2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Fonction: Rabbit IgG polyclonal antibody for DNA mismatch repair protein Msh2(MSH2) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for DNA mismatch repair protein Msh2(MSH2) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: mutS homolog 2
Protein Name: DNA mismatch repair protein Msh2

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human MSH2 recombinant protein (Position: Q337-N583). Human MSH2 shares 94% and 93% amino acid (aa) sequence identity with mouse and rat MSH2, respectively.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, The detection limit for MSH2 is approximately 0.2 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Références pour anticorps anti-MSH2 (ABIN3043885)

Zhang, Yue, Wang, Li, Xin, Pang, Zheng, Xie: "Cisplatin upregulates MSH2 expression by reducing miR-21 to inhibit A549 cell growth." dans: Biomedicine & pharmacotherapy, Vol. 67, Issue 2, pp. 97-102, (2014) (PubMed).

Détails sur MSH2

Antigène: MSH2 (Mismatch Repair Protein 2 (MSH2))

Autre désignation: MSH2 (MSH2 Produits)

Synonymes: anticorps COCA1, anticorps FCC1, anticorps HNPCC, anticorps HNPCC1, anticorps LCFS2, anticorps AI788990, anticorps wu:fc06b02, anticorps wu:fc13e09, anticorps zgc:55333, anticorps ATMSH2, anticorps MUTS homolog 2, anticorps msh2, anticorps mutS homolog 2, anticorps mutS homolog 2 (E. coli), anticorps MUTS homolog 2, anticorps mutS homolog 2 L homeolog, anticorps MutS protein homolog 2, anticorps MSH2, anticorps Msh2, anticorps msh2, anticorps msh2.L

Sujet: DNA mismatch repair protein Msh2, also known as MutS protein homolog 2 or MSH2, is a protein that in humans is encoded by the MSH2 gene, which is located on chromosome 2. MSH2 is a tumor suppressor gene and more specifically a caretaker gene that codes for a DNA mismatch repair (MMR) protein, MSH2 which forms aheterodimer with MSH6 to make the human MutSα mismatch repair complex. It also dimerizes with MSH3 to form the MutSβ DNA repair complex. MSH2 is involved in many different forms of DNA repair, including transcription-coupled repair, homologous recombination, and base excision repair. It has been found that MSH2 may also be a coactivator of ESR1-dependent gene expression.

Synonyms: BAT26 antibody|COCA 1 antibody|COCA1 antibody|DNA mismatch repair protein Msh2 antibody|FCC 1 antibody|FCC1 antibody|hMSH2 antibody|HNPCC 1 antibody|HNPCC antibody|HNPCC1 antibody|LCFS2 antibody|MSH 2 antibody|Msh2 antibody|MSH2_HUMAN antibody|MutS homolog 2 antibody|MutS homolog 2 colon cancer nonpolyposis type 1 antibody|MutS protein homolog 2 antibody

ID gène: 4436

UniProt: P43246

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response