ROCK2 anticorps (AA 652-923)
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- Antigène Voir toutes ROCK2 Anticorps
- ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))
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Épitope
- AA 652-923
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Reactivité
- Humain, Souris, Rat
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp ROCK2 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Fonction
- Rabbit IgG polyclonal antibody for Rho-associated protein kinase 2(ROCK2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
- Réactivité croisée (Details)
- No cross reactivity with other proteins.
- Attributs du produit
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Rabbit IgG polyclonal antibody for Rho-associated protein kinase 2(ROCK2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: Rho-associated, coiled-coil containing protein kinase 2
Protein Name: Rho-associated protein kinase 2 - Purification
- Immunogen affinity purified.
- Immunogène
- E.coli-derived human ROCK2 recombinant protein (Position: E652-D923). Human ROCK2 shares 94.9% and 95.2% amino acid (aa) sequence identity with mouse and rat ROCK2, respectively.
- Isotype
- IgG
- Top Product
- Discover our top product ROCK2 Anticorps primaire
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- Indications d'application
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for ROCK2 is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. - Commentaires
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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ROCK2 mediates the proliferation of pulmonary arterial endothelial cells induced by hypoxia in the development of pulmonary arterial hypertension." dans: Experimental and therapeutic medicine, Vol. 11, Issue 6, pp. 2567-2572, (2016) (PubMed).
: "The role of Rho/Rho-kinase pathway and the neuroprotective effects of fasudil in chronic cerebral ischemia." dans: Neural regeneration research, Vol. 10, Issue 9, pp. 1441-9, (2015) (PubMed).
: "Fluoxetine inhibits monocrotaline-induced pulmonary arterial remodeling involved in inhibition of RhoA-Rho kinase and Akt signalling pathways in rats." dans: Canadian journal of physiology and pharmacology, Vol. 90, Issue 11, pp. 1506-15, (2012) (PubMed).
: "8,9-Epoxyeicosatrienoic acid analog protects pulmonary artery smooth muscle cells from apoptosis via ROCK pathway." dans: Experimental cell research, Vol. 316, Issue 14, pp. 2340-53, (2010) (PubMed).
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ROCK2 mediates the proliferation of pulmonary arterial endothelial cells induced by hypoxia in the development of pulmonary arterial hypertension." dans: Experimental and therapeutic medicine, Vol. 11, Issue 6, pp. 2567-2572, (2016) (PubMed).
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- Antigène
- ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))
- Autre désignation
- ROCK2 (ROCK2 Produits)
- Synonymes
- anticorps rocka-a, anticorps xROK, anticorps rock2, anticorps rok2, anticorps ROCK2, anticorps B230113H15Rik, anticorps ROKalpha, anticorps Rho-kinase, anticorps Rock-II, anticorps Rock2m, anticorps mKIAA0619, anticorps ROCK-II, anticorps ROK, anticorps Rho-associated, coiled-coil containing protein kinase 2 L homeolog, anticorps rho-associated, coiled-coil containing protein kinase 2a, anticorps Rho associated coiled-coil containing protein kinase 2, anticorps Rho-associated, coiled-coil containing protein kinase 2, anticorps Rho-associated coiled-coil containing protein kinase 2, anticorps rock2.L, anticorps rock2a, anticorps ROCK2, anticorps Rock2
- Sujet
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Rho-associated kinase (ROCK), including the ROCK-I and ROCK-II isoforms, is a protein kinase involved in signaling from Rho to actin cytoskeleton. Serine/threonine kinase ROCK II/Rho kinase, which is an isozyme of ROCK I, is one of the targets for the small GTPase Rho. ROCK II regulates the formation of actin stress fibers and focal adhesions, cytokinesis, smooth muscle contraction, and the activation of c-fos serum response element. Sequencing analysis has shown that human ROCK II contains 1388 amino acid residues with a calculated molecular mass of approximately 161 kDa. Fluorescence in situ hybridization analysis showed that the human ROCK II gene is located on chromosome 2p24. Thumkeo et al. concluded that ROCK-II is essential in inhibiting blood coagulation and maintaining blood flow in the endothelium-free labyrinth layer and that loss of ROCK-II leads to thrombus formation, placental dysfunction, intrauterine growth retardation, and fetal death.
Synonyms: coiled-coil-containing protein kinase 2 antibody|KIAA0619 antibody|p164 ROCK 2 antibody|p164 ROCK-2 antibody|Rho associated coiled coil containing protein kinase 2 antibody|Rho associated protein kinase 2 antibody|Rho associated, coiled coil containing protein kinase II antibody|Rho kinase 2 antibody|Rho-associated antibody|Rho-associated protein kinase 2 antibody|ROCK 2 antibody|Rock II antibody|Rock2 antibody|ROCK2_HUMAN antibody|Rock2m antibody|ROK alpha antibody|ROKalpha antibody - ID gène
- 9475
- UniProt
- O75116
- Pathways
- Dynamique des Microtubules, Signalisation WNT, Tube Formation
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