YBX1 anticorps (C-Term)

N° du produit ABIN3043966

Détail du produit anti-YBX1 anticorps

Antigène: YBX1 (Y Box Binding Protein 1 (YBX1))

Épitope: AA 293-320, C-Term

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp YBX1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Fonction: Rabbit IgG polyclonal antibody for Nuclease-sensitive element-binding protein 1(YBX1) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.

Séquence: ENPKPQDGKE TKAADPPAEN SSAPEAEQ

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for Nuclease-sensitive element-binding protein 1(YBX1) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.
Gene Name: Y box binding protein 1
Protein Name: Nuclease-sensitive element-binding protein 1

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human YB1 (293-320aa ENPKPQDGKETKAADPPAENSSAPEAEQ), identical to the related mouse and rat sequences.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for YB1 is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Détails sur YBX1

Antigène: YBX1 (Y Box Binding Protein 1 (YBX1))

Autre désignation: YBX1 (YBX1 Produits)

Synonymes: anticorps BP-8, anticorps CSDA2, anticorps CSDB, anticorps DBPB, anticorps MDR-NF1, anticorps NSEP-1, anticorps NSEP1, anticorps YB-1, anticorps YB1, anticorps Byb1, anticorps Cbfa, anticorps Dbpb, anticorps Ef1a, anticorps Msy1, anticorps Nsep1, anticorps Yb1, anticorps bp-8, anticorps csda2, anticorps csdb, anticorps dbpb, anticorps frgy1, anticorps mdr-nf1, anticorps nsep-1, anticorps nsep1, anticorps yb1, anticorps 1700102N10Rik, anticorps C79409, anticorps EF1A, anticorps MSY1, anticorps dbpB, anticorps mYB-1a, anticorps nuclease sensitive element binding protein 1, anticorps Y-box binding protein 1, anticorps Y box binding protein 1, anticorps Y-box binding protein 1 S homeolog, anticorps nuclease-sensitive element-binding protein 1, anticorps Y box protein 1, anticorps nsep1, anticorps YBX1, anticorps Ybx1, anticorps ybx1.S, anticorps LOC479011

Sujet: YBX1(Y box binding protein 1), commonly referred to as "YB-1" by researchers, is a human protein. YB1 binding has an absolute requirement for the CCAAT box and relative specificity for the Y box. It has a molecular mass of 35,414 and contains 18 % basic residues and putative nuclear localization signals. The YBX1 gene is mapped on 1p34.2. Ybx1 was highly expressed in mouse erythroid myeloid lymphoid clone-1 (EML), a hematopoietic precursor cell line, but that it was downregulated in myeloid progenitors and in Gmcsf-treated EML cells during myeloid differentiation. Ybx1 was expressed at high levels in myeloid leukemic cells at different developmental stages. Knockdown of YBX1 in a human leukemic cell line inhibited proliferation ability, induced apoptosis, and induced megakaryocytic differentiation in response to arsenic trioxide treatment. YBX1 is downregulated during myeloid differentiation and aberrant YBX1 expression in leukemic cells may contribute to leukemia development by blocking differentiation.

Synonyms: BP 8 antibody|CBF-A antibody|CCAAT binding transcription factor I subunit A antibody|CCAAT-binding transcription factor I subunit A antibody|CSDA2 antibody|CSDB antibody|DBPB antibody|DNA binding protein B antibody|DNA-binding protein B antibody|EFI-A antibody| Enhancer factor I subunit A antibody|MDR NF1 antibody|MGC104858 antibody|MGC110976 antibody|MGC117250 antibody|NSEP 1 antibody|NSEP1 antibody|Nuclease sensitive element binding protein 1 antibody|Nuclease-sensitive element-binding protein 1 antibody|p50 antibody|Q15905 antibody|Y-box binding protein 1 antibody|Y-box transcription factor antibody|Y-box-binding protein 1 antibody|YB 1 antibody|YB-1 antibody|YBOX1_HUMAN antibody| YBX 1 antibody|YBX1 antibody

ID gène: 4904

UniProt: P67809

Pathways: Regulation of Muscle Cell Differentiation