WISP1 anticorps (C-Term)

N° du produit ABIN3043974

Détail du produit anti-WISP1 anticorps

Antigène: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Épitope: AA 354-367, C-Term

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp WISP1 est non-conjugé

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Fonction: Rabbit IgG polyclonal antibody for WNT1-inducible-signaling pathway protein 1(WISP1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.

Séquence: ADLESYPDFS EIAN

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for WNT1-inducible-signaling pathway protein 1(WISP1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: WNT1 inducible signaling pathway protein 1
Protein Name: WNT1-inducible-signaling pathway protein 1

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human WISP1(354-367aa ADLESYPDFSEIAN), different from the related mouse and rat sequences by one amino acid.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur WISP1

Antigène: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Autre désignation: WISP1 (WISP1 Produits)

Synonymes: anticorps CCN4, anticorps WISP1c, anticorps WISP1i, anticorps WISP1tc, anticorps WISP-1, anticorps WISP1, anticorps si:ch211-38f10.2, anticorps AW146261, anticorps Elm1, anticorps zgc:172276, anticorps WNT1 inducible signaling pathway protein 1, anticorps WNT1 inducible signaling pathway protein 1a, anticorps WNT1-inducible-signaling pathway protein 1-like, anticorps WNT1 inducible signaling pathway protein 1b, anticorps WISP1, anticorps wisp1a, anticorps Wisp1, anticorps LOC100627044, anticorps wisp1b

Sujet: WISP1(WNT1-Inducible Signaling Pathway Protein 1), also known as CCN4, is a matricellular protein that in humans is encoded by the WISP1 gene. WISP1 is induced by WNT1 and belongs to the CCN family, which includes connective tissue growth factor, cysteine-rich-61, and nephroblastoma overexpressed(Tanaka et al., 2001). By use of radiation hybrid mapping panels, Pennica et al.(1998) mapped the WISP1 to chromosome 8q24.1-q24.3, roughly 4 Mb distal to MYC. Pennica et al.(1998) found that 2 distinct systems demonstrated that WISP induction was associated with expression of WNT1. WISP1 genomic DNA was amplified in colon cancer cell lines and in human colon tumors, and its RNA was overexpressed in 84 % of tumors examined compared with patient-matched normal mucosa.

Synonyms: CCN 4 antibody|CCN family member 4 antibody|CCN4 antibody|WISP 1 antibody|WISP 1c antibody|WISP 1i antibody|WISP 1tc antibody|WISP1c antibody|WISP1i antibody|WISP1tc antibody|WNT 1 induced secreted protein 1 antibody|Wnt 1 induced secreted protein antibody|WNT 1 inducible signaling pathway protein 1 antibody|Wnt 1 signaling pathway protein 1 antibody|Wnt-1 inducible signaling pathway protein 1 antibody|WNT1 induced secreted protein 1 antibody|Wnt1 induced secreted protein antibody|WNT1 inducible signaling pathway protein 1 antibody|Wnt1 signaling pathway protein 1 antibody|WNT1-inducible-signaling pathway protein 1 antibody

UniProt: O95388

Pathways: Signalisation WNT, Growth Factor Binding