FLI1 anticorps (N-Term)

N° du produit ABIN3043998

Détail du produit anti-FLI1 anticorps

Antigène: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Épitope: AA 54-73, N-Term

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp FLI1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for Friend leukemia integration 1 transcription factor(FLI1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Séquence: QQEWINQPVR VNVKREYDHM

Réactivité croisée (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Attributs du produit: Rabbit IgG polyclonal antibody for Friend leukemia integration 1 transcription factor(FLI1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: Friend leukemia virus integration 1
Protein Name: Friend leukemia integration 1 transcription factor

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of human FLI1(54-73aa QQEWINQPVRVNVKREYDHM), identical to the related rat and mouse sequences.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Date de péremption: 12 months

Références pour anticorps anti-FLI1 (ABIN3043998)

Choi, El-Hamdi, McGregor: "Endothelial Cell Infection by Guinea Pig Cytomegalovirus Is a Lytic or Persistent Infection Depending on Tissue Origin but Requires Viral Pentamer Complex and pp65 Tegument Protein." dans: Journal of virology, Vol. 96, Issue 17, pp. e0083122, (2022) (PubMed).

Détails sur FLI1

Antigène: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Autre désignation: FLI1 (FLI1 Produits)

Synonymes: anticorps EWSR2, anticorps SIC-1, anticorps FLI1, anticorps fli, anticorps Xfli, anticorps fli1, anticorps Xfli1, anticorps fli-1, anticorps Xfli-1, anticorps MGC121593, anticorps Fli-1, anticorps Sic1, anticorps cb855, anticorps wu:fc45b11, anticorps xlfli, anticorps Fli-1 proto-oncogene, ETS transcription factor, anticorps Fli-1 proto-oncogene, ETS transcription factor S homeolog, anticorps Friend leukemia integration 1, anticorps Fli-1 proto-oncogene, ETS transcription factor a, anticorps Fli-1 proto-oncogene, ETS transcription factor L homeolog, anticorps FLI1, anticorps Fli1, anticorps fli1.S, anticorps fli1, anticorps fli1a, anticorps fli1.L

Sujet: Friend leukemia integration 1 transcription factor, also known as Transcription factor ERGB or Proto-oncogene Fli-1 is a protein that in humans is encoded by the human FLI1 gene. The fli1 protooncogene encodes a member of the ETS family of winged helix-turn-helix transcription factors. This gene is mapped to 11q24.3. Fli-1 is activated through retroviral insertional mutagenesis in 90 % of F-MuLV-induced erythroleukemias. The constitutive activation of fli-1 in erythroblasts leads to a dramatic shift in the Epo/Epo-R signal transduction pathway, blocking erythroid differentiation, activating the Ras pathway, and resulting in massive Epo-independent proliferation of erythroblasts. These results suggest that Fli-1 overexpression in erythroblasts alters their responsiveness to Epo and triggers abnormal proliferation by switching the signaling event(s) associated with terminal differentiation to proliferation.

Synonyms: ERGB transcription factor antibody|Ewing Sarcoma breakpoint region 2 antibody|EWSR 2 antibody|EWSR2 antibody|FLI 1 antibody|FLI 1 proto oncogene antibody|FLI1 antibody|FLI1 EWS fusion gene antibody|FLI1 proto oncogene antibody|FLI1_HUMAN antibody|Friend leukemia integration 1 transcription factor antibody|Friend leukemia virus integration 1 antibody|Proto-oncogene Fli-1 antibody|SIC 1 antibody|SIC1 antibody|Transcription factor ERGB antibody|Viral integration region FLI1 antibody

UniProt: Q01543