PDK2 anticorps (C-Term)

N° du produit ABIN3044044

Détail du produit anti-PDK2 anticorps

Antigène: PDK2 (Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2))

Épitope: AA 379-392, C-Term

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PDK2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Fonction: Rabbit IgG polyclonal antibody for [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial(PDK2) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.

Séquence: WRHYQTIQEA GDWC

Réactivité croisée (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Attributs du produit: Rabbit IgG polyclonal antibody for [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial(PDK2) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: pyruvate dehydrogenase kinase, isozyme 2
Protein Name: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human PDK2(379-392aa WRHYQTIQEAGDWC), identical to the related rat and mouse sequences.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur PDK2

Antigène: PDK2 (Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2))

Autre désignation: PDK2 (PDK2 Produits)

Synonymes: anticorps PDHK2, anticorps PDKII, anticorps Pdk, anticorps zgc:56209, anticorps PDK2, anticorps pyruvate dehydrogenase kinase 2, anticorps pyruvate dehydrogenase kinase, isoenzyme 2, anticorps pyruvate dehydrogenase kinase, isozyme 2b, anticorps PDK2, anticorps Pdk2, anticorps pdk2b

Sujet: PDK2(Pyruvate Dehydrogenase Kinase Isoenzyme 2), is an enzyme that in humans is encoded by the PDK2 gene. To find human PDKs, Gudi et al.(1995) used oligonucleotide primers to screen a human liver cDNA library by PCR. The human PDK2 gene encodes a protein with a predicted molecular mass of 46,181 Da that shares 96 % identity with that of the rat PDK2 gene. Northern blot analysis showed that the PDK2 message was found in many tissues, with the highest level in heart and skeletal muscle, intermediate levels in brain, kidney, pancreas and liver, and low levels in placenta and lung.

Synonyms: [Pyruvate dehydrogenase[lipoamide]] kinase isozyme 2 antibody|mitochondrial antibody|PDHK2 antibody|PDK2 antibody|PDK2_HUMAN antibody|Pyruvate dehydrogenase kinase isoform 2 antibody|Pyruvate dehydrogenase kinase, isozyme 2 antibody|Pyruvate dehydrogenase lipoamide kinase isozyme 2, mitochondrial antibody

UniProt: Q15119

Pathways: Signalisation PI3K-Akt, Signalisation RTK, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, L'effet Warburg