TRAM1 anticorps (C-Term)

N° du produit ABIN3044049

Détail du produit anti-TRAM1 anticorps

Antigène: TRAM1 (Translocation Associated Membrane Protein 1 (TRAM1))

Épitope: AA 314-331, C-Term

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TRAM1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Fonction: Rabbit IgG polyclonal antibody for Translocating chain-associated membrane protein 1(TRAM1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.

Séquence: KFINFQLRRW REHSAFQA

Réactivité croisée (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Attributs du produit: Rabbit IgG polyclonal antibody for Translocating chain-associated membrane protein 1(TRAM1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: translocation associated membrane protein 1
Protein Name: Translocating chain-associated membrane protein 1

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human TRAM1(314-331aa KFINFQLRRWREHSAFQA), identical to the related rat and mouse sequences.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, The detection limit for TRAM1 is approximately 1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F)and ICC.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur TRAM1

Antigène: TRAM1 (Translocation Associated Membrane Protein 1 (TRAM1))

Autre désignation: TRAM1 (TRAM1 Produits)

Synonymes: anticorps PNAS8, anticorps TRAM, anticorps TRAMP, anticorps MGC69465, anticorps 1810049E02Rik, anticorps cb289, anticorps tcamp, anticorps tram, anticorps wu:fb67f10, anticorps wu:fc67b03, anticorps wu:fi09h09, anticorps tram1, anticorps tram1l1, anticorps translocation associated membrane protein 1, anticorps translocating chain-associating membrane protein 1, anticorps translocation associated membrane protein 1 L homeolog, anticorps TRAM1, anticorps Tram1, anticorps tram1, anticorps tram1.L

Sujet: TRAM1(Translocation-Associating Membrane Protein 1), By crosslinking and reconstitution of canine proteoliposomes, followed by microsequencing and PCR screening of canine kidney and HeLa cell cDNA libraries, Gorlich et al.(1992) isolated cDNAs encoding TRAM(translocating chain-associating membrane protein). The International Radiation Hybrid Mapping Consortium mapped the TRAM gene to chromosome 8. Sequence analysis predicted that human TRAM is a 374-amino acid, 8-pass transmembrane protein that shares 95 % amino acid identity with the canine protein. Functional analysis indicated that TRAM influences glycosylation and is stimulatory or required for the translocation of secretory proteins.

Synonyms: PNAS 8 antibody|PNAS8 antibody|PRO1292 antibody|TRAM 1 antibody|TRAM antibody|TRAMP antibody|Translocating chain associated membrane protein 1 antibody|Translocating chain associating membrane protein antibody|Translocation associated membrane protein 1 antibody|Translocation associating membrane protein 1 antibody

UniProt: Q15629