MCM7 anticorps (C-Term)

N° du produit ABIN3044178

Détail du produit anti-MCM7 anticorps

Antigène: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Épitope: AA 699-719, C-Term

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MCM7 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Fonction: Rabbit IgG polyclonal antibody for DNA replication licensing factor MCM7(MCM7) detection. Tested with WB, IHC-P, ICC in Human.

Séquence: DEYEELNVWQ VNASRTRITF V

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for DNA replication licensing factor MCM7(MCM7) detection. Tested with WB, IHC-P, ICC in Human.
Gene Name: minichromosome maintenance complex component 7
Protein Name: DNA replication licensing factor MCM7

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human MCM7(699-719aa DEYEELNVWQVNASRTRITFV).

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur MCM7

Antigène: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Autre désignation: MCM7 (MCM7 Produits)

Synonymes: anticorps CDC47, anticorps MCM2, anticorps P1.1-MCM3, anticorps P1CDC47, anticorps P85MCM, anticorps PNAS146, anticorps MCM7, anticorps AI747533, anticorps Mcmd7, anticorps mCDC47, anticorps chunp6911, anticorps nyz175, anticorps sr:nyz175, anticorps cdc47, anticorps cdc47-2, anticorps mcm7, anticorps xmcm7, anticorps CG4978, anticorps DmMCM7, anticorps DmeMCM7, anticorps Dmel\\CG4978, anticorps McM7, anticorps Mcp-PCR1, anticorps PCR1, anticorps anon-EST:Liang-1.66, anticorps clone 1.66, anticorps minichromosome maintenance complex component 7, anticorps mini-chromosome maintenance complex protein 7, anticorps minichromosome maintenance complex component 2, anticorps minichromosome maintenance complex component 7 L homeolog, anticorps Minichromosome maintenance 7, anticorps minichromosome maintenance complex component 7 S homeolog, anticorps MCM complex subunit Mcm7, anticorps MCM7, anticorps CDC47, anticorps Mcm7, anticorps MCM2, anticorps mcm7, anticorps mcm7.L, anticorps mcm7.S

Sujet: MCM7(Minichromosome Maintenance, s. Cerevisiae, homolog of, 7), also called CDC47, FORMERLY, is one of the highly conserved mini-chromosome maintenance proteins(MCM) that are essential for the initiation of eukaryotic genome replication. The MCM7 gene is mapped on 7q22.1. MCM7 plays a pivotal role in the G1/S phase transition, orchestrating the correct assembly of replication forks on chromosomal DNA and ensuring that all the genome is replicated once and not more than once at each cell cycle. The MCM7 gene contains 15 exons. The miRNAs MIR106B, MIR93, and MIR25 are clustered in a 5-prime to 3-prime orientation within intron 13. Petrocca et al.(2008) found that MCM7 and the precursors of microRNAs(miRNAs) MIR106B, MIR93, and MIR25, all of which arise from intron 13 of the MCM7 gene, were overexpressed with almost perfect correlation in 5 of 10 human gastric tumors.

Synonyms: CDABP0042 antibody|CDC 47 antibody|CDC47 antibody|CDC47 homolog antibody|Cdc47, S. cerevisiae, homolog of antibody|DNA replication licensing factor MCM7 antibody|Homolog of S. cerevisiae Cdc47 antibody|MCM 2 antibody|MCM 7 antibody|MCM2 antibody|MCM2, formerly antibody|Mcm7 antibody|MCM7 minichromosome maintenance deficient 7 antibody|MCM7_HUMAN antibody|Minichromosome Maintainence 7 antibody|Minichromosome maintainence, S. cerevisiae, homolog of antibody|Minichromosome maintenance complex component 7 antibody|Minichromosome maintenance deficient 7 antibody|Minichromosome maintenance protein 7 antibody|P1.1 MCM3 antibody|P1.1-MCM3 antibody|P1CDC47 antibody|P85MCM antibody|PNAS 146 antibody|PNAS146 antibody

UniProt: P33993

Pathways: Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA