MCM2 anticorps (C-Term)

N° du produit ABIN3044242

Détail du produit anti-MCM2 anticorps

Antigène: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Épitope: AA 889-904, C-Term

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MCM2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Fonction: Rabbit IgG polyclonal antibody for DNA replication licensing factor MCM2(MCM2) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.

Séquence: NKFSHDLKRK MILQQF

Réactivité croisée (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Attributs du produit: Rabbit IgG polyclonal antibody for DNA replication licensing factor MCM2(MCM2) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: minichromosome maintenance complex component 2
Protein Name: DNA replication licensing factor MCM2

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human MCM2(889-904aa NKFSHDLKRKMILQQF), different from the related rat and mouse sequences by two amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur MCM2

Antigène: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Autre désignation: MCM2 (MCM2 Produits)

Synonymes: anticorps CG7538, anticorps DmMCM2, anticorps DmMcm2, anticorps DmeMCM2, anticorps Dmel\\CG7538, anticorps MCM2, anticorps MCM2-7, anticorps MCM2_DROME, anticorps McM2, anticorps PCR3, anticorps dMCM2, anticorps l(3)rL074, anticorps XMcm2, anticorps bm28, anticorps ccnl1, anticorps cdc19, anticorps cdcl1, anticorps mitotin, anticorps cb737, anticorps chunp6905, anticorps BM28, anticorps CCNL1, anticorps CDCL1, anticorps D3S3194, anticorps MITOTIN, anticorps MCM7, anticorps AA959861, anticorps AW476101, anticorps Mcmd2, anticorps mKIAA0030, anticorps Minichromosome maintenance 2, anticorps minichromosome maintenance complex component 2, anticorps minichromosome maintenance complex component 2 L homeolog, anticorps Mcm2, anticorps mcm2, anticorps MCM2, anticorps mcm2.L

Sujet: MCM2(MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 2),also known as MITOTIN, CDCL1 or BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division. And it is similar to members of the family of early S-phase proteins. The MCM2 gene is mapped to 3q21.3. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex(pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. In the G0 stage, the MCM2 and MCM5 proteins were much less abundant than the MCM7 and MCM3 proteins, which suggests that the MCM proteins are not present in stoichiometric amounts and that only a proportion of these molecules actively participate in cell cycle regulation as part of MCM complexes.

Synonyms: BM28 antibody|CCNL 1 antibody|CCNL1 antibody|CDC like 1 antibody|CDC like-1 antibody|cdc19 antibody|CDCL 1 antibody|CDCL1 antibody|Cell devision cycle like 1 antibody|Cyclin like 1 antibody|cyclin like-1 antibody|D3S3194 antibody|DNA replication licensing factor MCM2 antibody|KIAA0030 antibody|MCM 2 antibody|MCM2 antibody|MCM2 minichromosome maintenance deficient 2 mitotin antibody|MCM2 minichromosome maintenance deficient 2 mitotin(S. cerevisiae) antibody|MCM2 minichromosome maintenance deficient 2, mitotin antibody|MCM2_HUMAN antibody|MGC10606 antibody|Minichromosome maintenance complex component 2 antibody|Minichromosome maintenance deficient 2(mitotin) antibody|Minichromosome maintenance deficient 2 mitotin antibody|Minichromosome maintenance protein 2 antibody|Minichromosome maintenance protein 2 homolog antibody|Mitotin antibody|Nuclear protein BM28 antibody|OTTHUMP00000216047 antibody|OTTHUMP00000216050 antibody

UniProt: P49736

Pathways: Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA