MCM3 anticorps (N-Term)

N° du produit ABIN3044243

L’anticorps anti-MCM3 Polyclonal Lapin est utilisé pour la détection de MCM3 dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, IHC, IF, ICC et FACS.

Aperçu rapide pour MCM3 anticorps (N-Term) (ABIN3044243)

Antigène: MCM3 (Minichromosome Maintenance Complex Component 3 (MCM3))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MCM3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-MCM3 anticorps

Épitope: AA 13-27, N-Term

Fonction: Anti-MCM3 Antibody

Séquence: REAQRDYLDF LDDEE

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-MCM3 Antibody (ABIN3044243). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of human MCM3, identical to the related rat and mouse sequences.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur MCM3

Antigène: MCM3 (Minichromosome Maintenance Complex Component 3 (MCM3))

Autre désignation: MCM3

Sujet:

Synonyms: DNA replication licensing factor MCM3,3.6.4.12,DNA polymerase alpha holoenzyme-associated protein P1,P1-MCM3,RLF subunit beta,p102,MCM3,

Tissue Specificity: Heart, liver, duodenum, blood vessels and kidney.

Background: MCM3 (MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 3), also called RLFB or P1 PROTEIN, is a protein that in humans is encoded by the MCM3 gene. MCM3 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The MCM3 gene is mapped to 6p12.2. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact ly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.

Sequence Similarities: Belongs to the MCM family.

Poids moléculaire: 100-110 kDa

UniProt: P25205

Pathways: Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA