CYBB anticorps (Middle Region)

N° du produit ABIN3044252

Détail du produit anti-CYBB anticorps

Antigène: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Épitope: AA 442-459, Middle Region

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CYBB est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for Cytochrome b-245 heavy chain(CYBB) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Séquence: YWLCRDTHAF EWFADLLQ

Réactivité croisée (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Attributs du produit: Rabbit IgG polyclonal antibody for Cytochrome b-245 heavy chain(CYBB) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: cytochrome b-245, beta polypeptide
Protein Name: Cytochrome b-245 heavy chain

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence in the middle region of human NOX2(442-459aa YWLCRDTHAFEWFADLLQ), identical to the related rat and mouse sequences.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Date de péremption: 12 months

Références pour anticorps anti-CYBB (ABIN3044252)

Zhang, Deng, Lai, Guan, Sun, Han, Wang, Pan, Ji, Luo, Huang, Tang, Gu, Dan, Yu, Namaka, Zhang, Deng, Li: "Maternal inflammation activated ROS-p38 MAPK predisposes offspring to heart damages caused by isoproterenol via augmenting ROS generation." dans: Scientific reports, Vol. 6, pp. 30146, (2018) (PubMed).

Deep, Kumar, Jain, Dhar, Panigrahi, Hussain, Agarwal, El-Elimat, Sica, Oberlies, Agarwal: "Graviola inhibits hypoxia-induced NADPH oxidase activity in prostate cancer cells reducing their proliferation and clonogenicity." dans: Scientific reports, Vol. 6, pp. 23135, (2017) (PubMed).

Meyer, Fredette, Daniel, Sharma, Amann, Arterburn, Barton, Prossnitz: "Obligatory role for GPER in cardiovascular aging and disease." dans: Science signaling, Vol. 9, Issue 452, pp. ra105, (2017) (PubMed).

Wang, Wang, Liu, Wang, Zhao, Wang, Yin: "Cordyceps sinensis polysaccharide inhibits PDGF-BB-induced inflammation and ROS production in human mesangial cells." dans: Carbohydrate polymers, Vol. 125, pp. 135-45, (2015) (PubMed).

Du, Yang, Liu, Li, Zhao, Hu, Liu, Qian, Gao: "NADPH oxidase 2-dependent oxidative stress, mitochondrial damage and apoptosis in the ventral cochlear nucleus of D-galactose-induced aging rats." dans: Neuroscience, Vol. 286, pp. 281-92, (2015) (PubMed).

Du, Yang, Zhou, Liu, Li, Chen, Gao: "D?galactose?induced mitochondrial DNA oxidative damage in the auditory cortex of rats." dans: Molecular medicine reports, Vol. 10, Issue 6, pp. 2861-7, (2014) (PubMed).

Liao, Liu, Guo, Zhang, Zhang: "Oxidative burst of circulating neutrophils following traumatic brain injury in human." dans: PLoS ONE, Vol. 8, Issue 7, pp. e68963, (2013) (PubMed).

Détails sur CYBB

Antigène: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Autre désignation: CYBB (CYBB Produits)

Synonymes: anticorps zgc:64144, anticorps cgd, anticorps gp91-1, anticorps gp91-phox, anticorps gp91phox, anticorps nox2, anticorps NOX2, anticorps cybb, anticorps NOX1, anticorps AMCBX2, anticorps CGD, anticorps GP91-1, anticorps GP91-PHOX, anticorps GP91PHOX, anticorps p91-PHOX, anticorps Nox2, anticorps C88302, anticorps CGD91-phox, anticorps Cgd, anticorps Cyd, anticorps Gp91-phox, anticorps CYBB, anticorps cytochrome b-245, beta polypeptide (chronic granulomatous disease), anticorps cytochrome b-245, beta polypeptide, anticorps cytochrome b-245 heavy chain, anticorps cytochrome b-245, beta polypeptide L homeolog, anticorps cytochrome b-245 beta polypeptide, anticorps cytochrome b-245 beta chain, anticorps NADPH oxidase heavy chain subunit, anticorps cybb, anticorps CYBB, anticorps cybb.L, anticorps Cybb, anticorps GP91-PHOX

Sujet: NOX2(NADPH OXIDASE 2), also called CYBB(CYTOCHROME b(-245), BETA SUBUNIT), p91-PHOX or GP91-1, is a human gene encoding a glycoprotein.NOX2 is an essential component of phagocytic NADPH-oxidase, a membrane-bound enzyme complex that generates large quantities of microbicidal superoxide and other oxidants upon activation. It is mapped on Xp11.4. NOX2 is a heterodimer composed of an alpha chain of relative molecular mass 23 kD and a beta chain of 76 to 82 kD. NOX2 assembled on DC phagosomes in a gp91-phox subunit-dependent manner, and that reactive oxygen species were produced in a more sustained manner in immature DC phagosomes than in macrophage phagosomes. As a major player in innate immune responses in neutrophils, NOX2 is also involved in adaptive immunity through its activity in DCs. In heart cells, physiologic stretch rapidly activates reduced-form NOX2 to produce reactive oxygen species(ROS) in a process dependent on microtubules(X-ROS signaling).

Synonyms: AMCBX2 antibody|CGD antibody|CGD91-phox antibody|CY24B_HUMAN antibody|CYBB antibody|Cytochrome b 245, beta polypeptide antibody|Cytochrome b(558) beta chain antibody|Cytochrome b(558) subunit beta antibody|Cytochrome b-245 heavy chain antibody|Cytochrome b558 subunit beta antibody|GP91 PHOX antibody|gp91-1 antibody|gp91-phox antibody|GP91PHOX antibody|Heme-binding membrane glycoprotein gp91phox antibody|NADPH oxidase 2 antibody|Neutrophil cytochrome b 91 kDa polypeptide antibody|NOX2 antibody|p22 phagocyte B-cytochrome antibody|P91 PHOX antibody|p91-PHOX antibody|Superoxide-generating NADPH oxidase heavy chain subunit antibody

UniProt: P04839