CD68 anticorps (Middle Region)

N° du produit ABIN3044428

Détail du produit anti-CD68 anticorps

Antigène: CD68 (CD68 Molecule (CD68))

Épitope: AA 312-326, Middle Region

Reactivité: Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CD68 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Fonction: Rabbit IgG polyclonal antibody for Macrosialin(CD68) detection. Tested with WB, IHC-P, IHC-F in Mouse,Rat.

Séquence: AFCITRRRQS TYQPL

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for Macrosialin(CD68) detection. Tested with WB, IHC-P, IHC-F in Mouse,Rat.
Gene Name: CD68 Molecule
Protein Name: Macrosialin

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence in the middle region of mouse CD68(312-326aa AFCITRRRQSTYQPL), different from the related rat sequence by one amino acid.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Références pour anticorps anti-CD68 (ABIN3044428)

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Hoffman, Adeli: "LDL Receptor Gene-Ablated Hamsters: A Rodent Model of Familial Hypercholesterolemia with Dominant Inheritance and Diet-Induced Coronary Atherosclerosis." dans: EBioMedicine, Vol. 28, pp. 17-18, (2018) (PubMed).

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Guo, Liao, Huang, Wang, Huang, Liu: "Reversal of adipose tissue loss by probucol in mice with deficiency of both scavenger receptor class B type 1 and LDL receptor on high fat diet." dans: Biochemical and biophysical research communications, (2017) (PubMed).

Wang, Liu, Xiong, Xu, Shi, Qiu, Zang, Mao, Zhuang: "Inhibition of EGF Receptor Blocks the Development and Progression of Peritoneal Fibrosis." dans: Journal of the American Society of Nephrology : JASN, Vol. 27, Issue 9, pp. 2631-44, (2017) (PubMed).

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Leftin, Zhao, Turkekul, de Stanchina, Manova, Koutcher: "Iron deposition is associated with differential macrophage infiltration and therapeutic response to iron chelation in prostate cancer." dans: Scientific reports, Vol. 7, Issue 1, pp. 11632, (2017) (PubMed).

Huang, Zhang, Guo, Huang, Yang, Chen, Huang, Zhang, Long, Yang: "Honokiol protects against doxorubicin cardiotoxicity via improving mitochondrial function in mouse hearts." dans: Scientific reports, Vol. 7, Issue 1, pp. 11989, (2017) (PubMed).

Wang, Tian, Chen, Tan, Xie, Chen, Fu, Chen, Xu, Wei: "Low dose doxycycline decreases systemic inflammation and improves glycemic control, lipid profiles, and islet morphology and function in db/db mice." dans: Scientific reports, Vol. 7, Issue 1, pp. 14707, (2017) (PubMed).

Qu, Bing, Meng, Xi, Bai, Liu, Guo, Zhao, Bi: "Upregulation of miR-126-3p promotes human saphenous vein endothelial cell proliferation in vitro and prevents vein graft neointimal formation ex vivo and in vivo." dans: Oncotarget, Vol. 8, Issue 63, pp. 106790-106806, (2017) (PubMed).

Wang, Zhang, Chai, Liu, Li, Wang, Zhu, Cui, Qu, Zhu: "1,25(OH)2D3 downregulates the Toll-like receptor 4-mediated inflammatory pathway and ameliorates liver injury in diabetic rats." dans: Journal of endocrinological investigation, Vol. 38, Issue 10, pp. 1083-91, (2016) (PubMed).

Yan, Fan, Bai, Hou, Li, Tong: "Zinc Prevents Abdominal Aortic Aneurysm Formation by Induction of A20-Mediated Suppression of NF-κB Pathway." dans: PLoS ONE, Vol. 11, Issue 2, pp. e0148536, (2016) (PubMed).

Détails sur CD68

Antigène: CD68 (CD68 Molecule (CD68))

Autre désignation: CD68 (CD68 Produits)

Synonymes: anticorps Lamp4, anticorps Scard1, anticorps gp110, anticorps GP110, anticorps LAMP4, anticorps SCARD1, anticorps CD68 molecule, anticorps CD68 antigen, anticorps Cd68 molecule, anticorps CD68, anticorps Cd68

Sujet: CD68, cluster of differentiation, is a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. CD68 is a member of a family of hematopoietic mucin-like molecules that includes leukosialin/CD43 and stem cell antigen CD34. The CD68 gene is mapped to 17p13.1. Immunohistochemistry can be used to identify the presence of CD68, which is found in the cytoplasmic granules of a range of different blood cells. It is particularly useful as a marker for the various cells of the macrophage lineage, including monocytes, histiocytes, giant cells, Kupffer cells, and osteoclasts. This allows it to be used to distinguish diseases of otherwise similar appearance, such as the monocyte/macrophage and lymphoid forms of leukaemia(the latter being CD68 negative). Its presence in macrophages also makes it useful in diagnosing conditions related to proliferation or abnormality of these cells, such as malignant histiocytosis, histiocytic lymphoma, and Gaucher's disease.

Synonyms: CD 68 antibody|CD68 antibody|CD68 antigen antibody|CD68 Molecule antibody|CD68_HUMAN antibody|DKFZp686M18236 antibody|GP110 antibody|LAMP4 antibody|Macrophage antigen CD68(microsialin) antibody|MACROPHAGE ANTIGEN CD68 antibody|macrosialin antibody|SCARD1 antibody|Scavenger receptor class D member 1 antibody

UniProt: P31996