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ZP2 anticorps (N-Term)

ZP2 Reactivité: Humain WB, IHC (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN3044563
  • Antigène Voir toutes ZP2 Anticorps
    ZP2 (Zona Pellucida Glycoprotein 2 (ZP2))
    Épitope
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 511-544, N-Term
    Reactivité
    • 21
    • 5
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 19
    • 4
    Lapin
    Clonalité
    • 20
    • 3
    Polyclonal
    Conjugué
    • 14
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp ZP2 est non-conjugé
    Application
    • 12
    • 6
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Fonction
    Rabbit IgG polyclonal antibody for Zona pellucida sperm-binding protein 2(ZP2) detection. Tested with WB, IHC-P, IHC-F in Human.
    Séquence
    ENEYPLVRFL RQPIYMEVRV LNRDDPNIKL VLDD
    Réactivité croisée (Details)
    No cross reactivity with other proteins.
    Attributs du produit
    Rabbit IgG polyclonal antibody for Zona pellucida sperm-binding protein 2(ZP2) detection. Tested with WB, IHC-P, IHC-F in Human.
    Gene Name: zona pellucida glycoprotein 2 (sperm receptor)
    Protein Name: Zona pellucida sperm-binding protein 2
    Purification
    Immunogen affinity purified.
    Immunogène
    A synthetic peptide corresponding to a sequence at the N-terminus of human ZP2 (511-544aa ENEYPLVRFLRQPIYMEVRVLNRDDPNIKLVLDD), different from the related mouse sequence by six amino acids, and from the related rat sequence by four amino acids.
    Isotype
    IgG
    Top Product
    Discover our top product ZP2 Anticorps primaire
  • Indications d'application
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
    IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
    IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Human
    Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
    Commentaires

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Concentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeated freezing and thawing.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
  • Antigène
    ZP2 (Zona Pellucida Glycoprotein 2 (ZP2))
    Autre désignation
    ZP2 (ZP2 Produits)
    Synonymes
    anticorps fa12e07, anticorps wu:fa12e07, anticorps zgc:152728, anticorps zp2, anticorps Zp-2, anticorps ZPA, anticorps zona pellucida glycoprotein 2, tandem duplicate 5, anticorps zona pellucida glycoprotein 2, anticorps zp2.5, anticorps Zp2, anticorps ZP2
    Sujet
    Zona pellucida sperm-binding protein 2 is a protein that in humans is encoded by the ZP2 gene. The sperm-binding domain on the ZP2 protein is necessary in both humans and mice for oocyte-sperm recognition and penetration of the zona pellucida. It is also responsible for the primary block to polyspermy in mammals. The oocyte has cortical granules peripherally located under the cortex that contain a proteolytic protein called ovastacin. After the sperm binds to ZP2, the cortical granules are exocytosed releasing ovastacin into the perivitelline space. Ovastacin cleaves ZP2 at the N terminus, preventing more sperm from binding and penetrating the oocyte, thus hardening the zona pellucida. Ovastacin is only found in oocytes, and is part of the astacin family of metalloendoproteases. Female mice engineered without ovastacin showed that ZP2 was not cleaved after fertilization.

    Synonyms: OTTHUMP00000115849 antibody|Processed zona pellucida sperm-binding protein 2 antibody|Zona pellucida glycoprotein 2 antibody|zona pellucida glycoprotein ZP2 antibody|Zona pellucida protein A antibody|Zp-2 antibody|ZP2 antibody|ZP2_HUMAN antibody|ZPA antibody
    ID gène
    7783
    UniProt
    Q05996
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