TCR gamma/delta anticorps (FITC)

N° du produit ABIN3044601

Détail du produit anti-TCR gamma/delta anticorps (FITC)

Antigène: TCR gamma/delta (T-Cell Receptor gamma/delta (TCR gamma/delta))

Reactivité: Veuillez nous consulter SVP

Hôte: Hamster arménien

Clonalité: Monoclonal

Conjugué: Cet anticorp TCR gamma/delta est conjugé à/à la FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Immunogène: Proprietary Immunogen Species

Clone: UC7-13D5

Isotype: IgG

Information d'application

Indications d'application: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of mouse tissue for flow cytometric analysis using 1 µg/10^6 cells

Commentaires:

Fluorescein Isothiocyanate, FITC (Abs/Em: 495/ 517 nm).

Préparation des réactifs:

1. Transfer the sample to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add anti-mouse anti-mouse TCR gamma/delta FITC and mix gently with a vortex mixer. The optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive FITC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 mL of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 mL 0.01 mol/L PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 mL PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.

Agent conservateur: Sodium azide

Précaution d'utilisation: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Stock: 4 °C

Références pour anticorps anti-TCR gamma/delta (FITC) (ABIN3044601)

Whiteland, Nicholls, Shimeld, Easty, Williams, Hill: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." dans: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, (1995) (PubMed).

Détails sur TCR gamma/delta

Antigène: TCR gamma/delta (T-Cell Receptor gamma/delta (TCR gamma/delta))

Autre désignation: TCR gamma/delta (TCR gamma/delta Produits)

Sujet: T cell receptor (TCR) is a heterodimer consisting of an alpha and a beta chain (TCR alpha/beta) or a gamma and a delta chain (TCR gama/delta). TCR-beta is a member of the immunoglobulin superfamily and a component of the CD3/TCR complex (along with TCR-alpha). It is expressed on alpha/beta TCR-bearing T cells and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation.