H-2Dd anticorps (FITC)

N° du produit ABIN3044604

Détail du produit anti-H-2Dd anticorps (FITC)

Antigène: H-2Dd (MHC Class I H-2Dd)

Reactivité: Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp H-2Dd est conjugé à/à la FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Attributs du produit: The 34-2-12 monoclonal antibody reacts with the mouse alpha3 domain of H-2Dd MHC class I alloantigen expressed on nucleated cells from mice of the H-2Dd haplotype.

Immunogène: C57BL/6 x DBA/2)F1 mouse splenocytes

Clone: 34-2-12

Isotype: IgG2a

Information d'application

Indications d'application: It is recommended for use in flow cytometry. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent iless or equal to 0.25 µg per 10^6 cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Commentaires:

Fluorescein isothiocyanate (FITC). Abs/Em: 494/519 nm.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing 0,09% Sodium azide, pH 7.2.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Détails sur H-2Dd

Antigène: H-2Dd (MHC Class I H-2Dd)

Autre désignation: H-2Dd (H-2Dd Produits)

Sujet: The 34-2-12 antibody (also known as 34-2-12S) recognizes the alpha3 domain of the H-2D^[d]. The binding of the antibody to its epitope is independent of the alpha1 and alpha2 domains and beta2 microglobulin. It cross-reacts with cells of the C3H.LG/Ckc strain. Reactivity with other haplotypes (eg, b, f, k, p, q, r, s) has not been observed. Soluble mAb 34-2-12 blocks binding of the Ly-49A-expressing T lymphoma EL4 to immobilized H-2D[d]. However, further studies utilizing this mAb indicate that the alpha3 domain is not involved in the interaction between Ly-49A, or Ly-49G2, and H-2D[d].