Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Réactivité croisée
Rat
Attributs du produit
Eph B1 (ELK receptor) SAM, Tyrosine-protein kinase receptor EPH-2, Ephrin type B receptor 1, HEK6,Activation of protein kinase C is a key signal transduction event in mesangial cell dedifferentiation and proliferation, yet little is known about downstream substrates or their roles in normal or diseased states. SSeCKS, a novel protein kinase C substrate originally isolated as a src-suppressed negative mitogenic regulator in fibroblasts, controls actinbased cytoskeletal architecture and scaffolds key signaling kinases such as protein kinase C and protein kinase A. Activation of protein kinase C is a key signal transduction event in mesangial cell dedifferentiation. A role for SSeCKS, a PKA/PKC scaffolding protein, has been implicated during the process of spermiogenesis and in the actin-based stellate morphology of mesangial cells
Purification
Ammonium Sulfate Precipitation
Immunogène
Sheep were immunized with a recombinant protein corresponding to amino acids 620-1596 of the SSeCKS protein.
EPHB1
Reactivité: Souris, Rat
WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro)
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Antibody can be used for Western blot (2-5 μg/mL), immunohistochemistry and immunoprecipitation. Optimal concentration should be evaluated by serial dilutions.
Restrictions
For Research Use only
Buffer
Provided as solution in phosphate buffered saline with 0.08 % sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C
Stockage commentaire
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles