Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
Réactivité croisée
Souris
Attributs du produit
Rho guanine nucleotide exchange factor 2, ARHGEF2,Lfc is a 118 kDa protein discovered in a screen for oncogenes in NIH 3T3 cells. Lfc is a signaling protein related to Lbc and is part of the Dbl family of proteins. Lfc is a guanine-nucleotide exchange factor (GEF) for the Rho family of small GTPases. Lfc has also been shown to be upstream of the serine-threonine kinase JNK. Lfc has also been shown to mediate the activation of the Rac signaling pathway.
Purification
Ammonium Sulfate Precipitation
Immunogène
Antibody developed using protein corresponding to amino acids 2 to 447 of the Lfc protein fused to GST.
Detects Lfc in mouse tissues by immunoprecipitation-Western blot at 4 μg/mL. Detects a 118 kDa band in mouse tissue extracts corresponding to wild-type Lfc. Can also be used in immunofluorescence assays. Optimal concentration should be evaluated by serial dilutions.
Restrictions
For Research Use only
Buffer
Provided as solution in phosphate buffered saline with 0.08 % sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C
Stockage commentaire
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles