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Luciferase anticorps

Reactivité: Photinus pyralis WB Hôte: Souris Monoclonal Luci17 unconjugated
N° du produit ABIN306791
  • Antigène Voir toutes Luciferase Anticorps
    Luciferase
    Reactivité
    • 32
    • 19
    • 15
    • 7
    • 4
    • 1
    • 1
    Photinus pyralis
    Hôte
    • 56
    • 13
    • 9
    • 1
    Souris
    Clonalité
    • 51
    • 28
    Monoclonal
    Conjugué
    • 32
    • 12
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Luciferase est non-conjugé
    Application
    • 38
    • 26
    • 15
    • 13
    • 13
    • 11
    • 11
    • 8
    • 7
    • 7
    • 4
    • 4
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB)
    Clone
    Luci17
    Isotype
    IgG1
    Top Product
    Discover our top product Luciferase Anticorps primaire
  • Indications d'application
    Detects luciferase protein by Western blot in C. elegans and Drosophilia melanogaster tissues, human fibroblast, mouse macrophage, kidney, liver and cortex as well as NIH3T3, Jurkat and BHR21 cell lines. Detects luciferase with little to no background signal. Positive Control: Purified luciferase protein.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/ml
    Stock
    -20 °C
  • Antigène
    Luciferase
    Abstract
    Luciferase Produits
    Synonymes
    anticorps hypothetical protein, anticorps luciferase, anticorps C25G6.3, anticorps Gbro_2186
    Sujet
    Analysis of gene expression is most commonly assayed by transient transfection. These systems are generally based on the use of fusion genes which are inserted into cells, and the gene expression is assayed within 48 hours after introduction of DNA. Usually the fusion consists of the promoter binding site or enhancer sequence under study which is attached to a reporter gene. The amount of the reporter protein synthesized under the experimental conditions, is presumed to reflect the ability of the sequences studied to direct or promote transcription. Several enzymes are commonly used as reporter proteins, among them are chloramphenicol acetyl transferase (CAT), -galactosidase, human growth hormone (hGH) and luciferase. Luciferase has become one of the widely used reporter enzymes. The enzyme catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg+2 and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample. The use of an antibody to detect luciferase can provide an alternative detection assay which directly detects luciferase protein levels, and thus has the advantage that it does not require luciferase activity and is not dependent on rapid kinetics. Moreover, antibodies can detect the luciferase enzyme expression in situ, providing a means to study the localized signal sequences using luciferase as a reporter gene.
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