RIT1 anticorps

N° du produit ABIN306804

Détail du produit anti-RIT1 anticorps

Antigène: RIT1 (Ras-Like Without CAAX 1 (RIT1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp RIT1 est non-conjugé

Application: Western Blotting (WB)

Réactivité croisée: Humain, Souris

Attributs du produit: Rit and its neuron-specific homologue, Rin, define a recently discovered subfamily of Ras-related GTPases. Rit and Rin are membrane-associated in spite of the fact that they lack a CAAX box or similar C-terminal lipidation motif. Rit and Rin display 64 % amino acid sequence identity and share a unique nine amino acid effector domain (DPTIEDAYK) that is 100 % conserved between the murine and human proteins. Although the effector domain sequences of Rit and Rin are very similar to that of Ras, Rit and Rin have been shown to interact with the known Ras-binding proteins RalGDS, Rlf and AF-6, but not the Raf kinases, RIN1 or the p110 subunit of PI3 kinase. For this reason, it has been suggested that Rit and Rin may play important roles in the regulation of signaling pathways distinct from those controlled by Ras.

Purification: Protein A or G purified

Immunogène: Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant full-length human Rit protein and mouse myeloma cells.

Clone: 14G7

Isotype: IgG1

Information d'application

Indications d'application: Antibody detects recombinant Rit protein expressed in 293 cells by Western blot analysis at a concentration of 0.5 to 1 μg/mL. Detects a band of approximately 25 kDa (predicted molecular weight: 26 kDa). Does not cross-react with Rin protein. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Stockage

Buffer: Provided as sterile filtered solution containing 20 mM sodium phosphate, 150 mM sodium chloride, 50 % glycerol at pH 7.5 and 3 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Détails sur RIT1

Antigène: RIT1 (Ras-Like Without CAAX 1 (RIT1))

Autre désignation: Rit (RIT1 Produits)

Synonymes: anticorps NS8, anticorps RIBB, anticorps RIT, anticorps ROC1, anticorps RIT1, anticorps rit1, anticorps MGC79097, anticorps RGD1559874, anticorps si:ch211-93a2.3, anticorps Rit, anticorps Ras like without CAAX 1, anticorps Ras-like without CAAX 1 L homeolog, anticorps Ras-like without CAAX 1, anticorps RIT1, anticorps rit1.L, anticorps Rit1, anticorps rit1

UniProt: Q92963

Pathways: Neurotrophin Signaling Pathway