SRPR anticorps (AA 39-295)

N° du produit ABIN307162

Détail du produit anti-SRPR anticorps

Antigène: SRPR (Signal Recognition Particle Receptor (Docking Protein) (SRPR))

Épitope: AA 39-295

Reactivité: Chien

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp SRPR est non-conjugé

Application: Western Blotting (WB)

Réactivité croisée: Chien

Attributs du produit: Signal Recognition Particle Receptor, α subunit,The receptor for Signal Recognition Particle (SRP) is the site on the endoplasmic reticulum that ribosomes translating secreted and integral membrane proteins are initially targeted to. Once the ribosome-SRP complex arrives at the SRP receptor the protein being translated is transferred to the translocation complex (Sec61) in the ER membrane. The SRP receptor is composed of two subunits SRalpha and SRbeta. The SRalpha subunit is a translocation GTPase peripherally bound to the endoplasmic reticulum by its interaction with SRbeta. SRalpha also binds to the GTPase of SRP (SRP54) and these two proteins appear to function as each others GTPase activating proteins (GAPs). Hydrolysis of GTP by SRalpha and SRP54 is thought to be involved in transfer of the nascent protein to the Sec61 complex in the ER. SRalpha has an apparent molecular weight of 72 kDa. SRbeta is a Type I transmembrane protein that spans the membrane once and contains Ras type GTPase domain. The function of the GTPase in SRbeta is unknown. The membrane spanning domain is at the amino-terminus of SRbeta. The GTPase domain encompasses three quarters of the protein and is carboxyl- of the transmembrane region. SRalpha binds to the GTPase domain of SRbeta. Heterodimerization of SRalpha and SRbeta masks the carboxyl-terminal epitope of SRbeta.

Purification: Ammonium Sulfate Precipitation

Immunogène: The antibody to SRalpha was raised against a recombinant protein corresponding to amino acids 39-295 which includes part of the amino terminal SRbeta binding region and the hinge region between it and the carboxyl-terminal GTPase domain.

Isotype: IgG

Information d'application

Indications d'application: Detects SRalpha by Western blot analysis at 1 to 5 μg/mL. Also can be used to immunoprecipitate both subunits of SRP receptors under non-denaturing conditions. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Stockage

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Détails sur SRPR

Antigène: SRPR (Signal Recognition Particle Receptor (Docking Protein) (SRPR))

Autre désignation: SR alpha (SRPR Produits)

Synonymes: anticorps sralpha, anticorps srp-alpha, anticorps 1300011P19Rik, anticorps D11Mgi27, anticorps DP, anticorps Sralpha, anticorps SRP receptor alpha subunit, anticorps signal recognition particle receptor (docking protein), anticorps signal recognition particle receptor subunit alpha, anticorps signal recognition particle receptor, anticorps signal recognition particle receptor (Docking protein), anticorps signal recognition particle receptor ('docking protein'), anticorps srpra, anticorps ftsY, anticorps Tb11.01.1650, anticorps STER_1399, anticorps CMU_011790, anticorps EUBELI_01057, anticorps MGYG_00278, anticorps Tsp_06333, anticorps Srpr, anticorps Srpra, anticorps SRPRA

Pathways: ER-Nucleus Signaling