HES1 anticorps (AA 24-35)

N° du produit ABIN307170

Détail du produit anti-HES1 anticorps

Antigène: HES1 (Hes Family bHLH Transcription Factor 1 (HES1))

Épitope: AA 24-35

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Lapin, Poisson zèbre (Danio rerio), Cheval, Singe, Roussette (Chauve-souris), Xenopus laevis, Hamster

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HES1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Marque: IHC-plus™

Specificité: Recognizes human HES-1 (Hairy family).

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Horse, Rabbit, Xenopus, Salmon, Stickleback, Pufferfish, Zebrafish (100%) Newt, Smelt (91%) Chicken (82%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide corresponding to aa24-35 (TPDKPKTASEH) OF HES1. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Horse, Rabbit, Xenopus, Salmon, Stickleback, Pufferfish, Zebrafish (100%), Newt, Smelt (91%), Chicken (82%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IF, IHC, IHC-Fr, IHC-P (5 μg/mL), WB (1:200 - 1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Positive control: Human neural stem cells, Raji cell lysate.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2. No preservative added.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Références pour anticorps anti-HES1 (ABIN307170)

Moya, Umans, Maas, Pereira, Beets, Francis, Sents, Robertson, Mummery, Huylebroeck, Zwijsen: "Stalk cell phenotype depends on integration of Notch and Smad1/5 signaling cascades." dans: Developmental cell, Vol. 22, Issue 3, pp. 501-14, (2012) (PubMed).

Détails sur HES1

Antigène: HES1 (Hes Family bHLH Transcription Factor 1 (HES1))

Autre désignation: HES1 / HES-1 (HES1 Produits)

Sujet: Name/Gene ID: HES1

Synonyms: HES1, BHLHb39, HHL, HL, Hairy and enhancer of split 1, Hairy homolog, HRY, Hairy homolog (Drosophila), Transcription factor HES-1, Hairy-like protein, HES-1

ID gène: 3280

UniProt: Q14469

Pathways: Réparation de l'ADN