5-Methylcytidine anticorps
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- Antigène Tous les produits 5-Methylcytidine
- 5-Methylcytidine
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp 5-Methylcytidine est non-conjugé
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- Specificité
- Modified base 5-methylcytidine
- Purification
- Purified IgG from culture supernatant by Affinity chromatography on Protein A ceramic hyper D F - Filtration on 0.22 µm.
- Clone
- 33D3
- Isotype
- IgG1
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- Indications d'application
- MeDIP: Used 5ul in 200ul reaction with 2ug digested genomic DNA from Drosophila. Flow Cyt: Use 10ul of working dilution to label 1000000 cells in 100ul. ICC/IF: Used at a dilution of 1/200 for 16 hrs. IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Sodium citrate buffer pH6.0 is recommended for this purpose. IHC-Fr: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
- Commentaires
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For research use only
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/ml
- Buffer
- Phosphate Buffered Saline 10 mM – NaCl 0.15 M – pH 7.4
- Stock
- -20 °C
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Functional analysis of male mouse haploid germ cells of various differentiation stages: early and late round spermatids are functionally equivalent in producing progeny." dans: Biology of reproduction, Vol. 80, Issue 3, pp. 511-7, (2009) (PubMed).
: "Evidence for transgenerational transmission of epigenetic tumor susceptibility in Drosophila." dans: PLoS genetics, Vol. 3, Issue 9, pp. 1598-606, (2007) (PubMed).
: "
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Functional analysis of male mouse haploid germ cells of various differentiation stages: early and late round spermatids are functionally equivalent in producing progeny." dans: Biology of reproduction, Vol. 80, Issue 3, pp. 511-7, (2009) (PubMed).
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- Antigène
- 5-Methylcytidine
- Abstract
- 5-Methylcytidine Produits
- Classe de substances
- Chemical
- Sujet
- DNA methylation is a DNA modification process, which is involved in the control of gene expression. The 5-Methylcytidine antibody specifically binds the modified base 5-methylcytidine (5-MeCyd) found in DNA of plants and vertebrates.
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