This antibody is specific for Porcine CD107a, a cell surface antigen that is also known as lysosomal-associated membrane protein-1 (LAMP-1). CD107a is expressed on macrophages and more weakly on monocytes and granulocytes.
Réactivité croisée (Details)
Species reactivity (tested):Pig.
Attributs du produit
Synonyms: LAMP-1, LAMPA, LGP120, Lysosome-associated membrane glycoprotein 1, CD107Antigen-like Family Member A
Purification
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogène
Porcine alveolar Macrophages. Spleen cells from immunised BALB/c mice were fused with cells of the mouseX63-Ag. 8. 653 myeloma cell line.
Flow Cytometry: Use 10 μL of Neat antibody to label 10^6 cells in 100 μL (Membranepermeabilisation is required). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.1 mg/mL
Buffer
PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Date de péremption
12 months
Antigène
LAMP1
(Lysosomal-Associated Membrane Protein 1 (LAMP1))
Lysosome associated membrane protein (LAMP1), also known as lgp120 or lgpA, is a type 1 integral membrane protein that is transported from trans Golgi networks to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboxyl terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1. This mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore they are possibly involved in the adhesion of PBMCs to the site of inflammation.Synonyms: CD107 Antigen-like Family Member A, LAMP-1, LAMPA, LGP120, Lysosome-associated membrane glycoprotein 1