TOP2A
Reactivité: Humain
WB, ELISA
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
ELISA: 1/1000-1/5000. Western Blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
anticorps TOP2, anticorps TP2A, anticorps TOP2A, anticorps Top-2, anticorps TOP-2, anticorps F12B17.110, anticorps F12B17_110, anticorps thimet metalloendopeptidase 2, anticorps fc08c07, anticorps wu:fc08c07, anticorps DNA topoisomerase II alpha, anticorps DNA topoisomerase II, alpha isozyme, anticorps topoisomerase (DNA) II alpha, anticorps Zincin-like metalloproteases family protein, anticorps TOP2A, anticorps Bm1_08705, anticorps Top2a, anticorps AT5G10540, anticorps top2a
Sujet
DNA topoisomerase I and II (Topo I and Topo II) are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Eukaryotic topoisomerases are capable of relaxing both positive and negative supercoils, whereas prokaryotic topoisomerases relax only negative supercoils. DNA topoisomerases play a role in DNA replication, recombination, and transcription and have been identified as targets of numerous anticancer drugs. Topo I, a ubiquitously expressed, soluble enzyme, acts by introducing a transient break in one strand of DNA, while Topo II acts by making a transient double-strand break. Topo II is encoded by two different genes to generate two distinct isoforms that are designated Topo IIalpha and Topo IIbeta. The 180 kDa isoform, Topo IIbeta, and the 170 kDa isoform, Topo IIalpha, are largely homologous at their N-terminal three quarters, however, the C-terminal segments are considerably divergent, suggesting that these regions may mediate different cellular functions and account for the observed differential tissue expression patterns of the two isoforms.Synonyms: DNA topoisomerase 2-alpha, DNA topoisomerase II alpha isozyme, TOP2, Top-2