SYK anticorps (pTyr323)
-
- Antigène Voir toutes SYK Anticorps
- SYK (Spleen tyrosine Kinase (SYK))
-
Épitope
- pTyr323
-
Reactivité
- Humain
-
Hôte
- Lapin
-
Clonalité
- Polyclonal
-
Conjugué
- Cet anticorp SYK est non-conjugé
-
Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificité
- Antibody AP08041PU detects endogenous levels of Syk only when phosphorylated at Tyrosine 323.
- Purification
- Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
- Immunogène
- The antiserum was produced against synthesized phosphopeptide derived from human syk around the phosphorylation site of Tyrosine 323 (N-P-YP-E- P).
- Top Product
- Discover our top product SYK Anticorps primaire
-
-
- Indications d'application
-
Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
-
- Concentration
- 1.0 mg/mL
- Buffer
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- -20 °C
- Stockage commentaire
- Store the antibody (in aliquots) at-20 °C.
-
- Antigène
- SYK (Spleen tyrosine Kinase (SYK))
- Autre désignation
- SYK (SYK Produits)
- Sujet
- Syk (72 kDa) is a non receptor protein tyrosine kinase that plays an important role in immune receptor signal transduction and is implicated in endothelial cell functions, including cell growth and migration. SYK is a positive effector of BCR stimulated responses. It couples the B cell antigen receptor (BCR) to the mobilization of calcium ions either through a phosphoinositide 3 kinase dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C gamma dependent pathway, when phosphorylated on Tyr 342 and Tyr 346. Thus the differential phosphorylation of SYK can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ions.Synonyms: Spleen tyrosine kinase, Tyrosine-protein kinase SYK
- ID gène
- 6850
- NCBI Accession
- NP_001128524
- UniProt
- P43405
- Pathways
- Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Thromboxane A2 Receptor Signaling, BCR Signaling
-