No cross-reactivity with: Chlamydomonas reinhardtii
Attributs du produit
Expected / apparent Molecular Weight of the Antigene: 36-40 / 36-40 for Arabidopsis thaliana
Purification
serum
Immunogène
KLH-conjugated synthetic peptide derived from fully conserved C-terminal consensus motif from plant AOX isoforms 1 and 2 including Arabidopsis thaliana AOX1A (At3g22370) and AOX2 (At3g64210), Solanum lycopersicum Q7XBG9, Oryza sativa Q7XT33,
Indications d'application
Recommended Dilution: 1 : 1000 on 10-20 µg of mitochondrial protein/lane with standard ECL detection(WB).
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
For reconstitution add 200 µL of sterile water
Conseil sur la manipulation
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Stock
-20 °C
Barreto, Okura, Neshich, Maia, Arruda: "Overexpression of UCP1 in tobacco induces mitochondrial biogenesis and amplifies a broad stress response." dans: BMC plant biology, Vol. 14, pp. 144, (2014) (PubMed).
Tsaniklidis, Delis, Nikoloudakis, Katinakis, Aivalakis: "Low temperature storage affects the ascorbic acid metabolism of cherry tomato fruits." dans: Plant physiology and biochemistry : PPB / Société française de physiologie végétale, Vol. 84, pp. 149-57, (2014) (PubMed).
Zubo, Potapova, Yamburenko, Tarasenko, Konstantinov, Börner: "Inhibition of the electron transport strongly affects transcription and transcript levels in Arabidopsis mitochondria." dans: Mitochondrion, Vol. 19 Pt B, pp. 222-30, (2014) (PubMed).
Wang, Vanlerberghe: "A lack of mitochondrial alternative oxidase compromises capacity to recover from severe drought stress." dans: Physiologia plantarum, (2013) (PubMed).
Kornfeld, Heskel, Atkin, Gough, Griffin, Horton, Turnbull: "Respiratory flexibility and efficiency are affected by simulated global change in Arctic plants." dans: The New phytologist, Vol. 197, Issue 4, pp. 1161-72, (2013) (PubMed).
Zhang, Peck: "Simplified enrichment of plasma membrane proteins for proteomic analyses in Arabidopsis thaliana." dans: Proteomics, Vol. 11, Issue 9, pp. 1780-8, (2011) (PubMed).
Wang, Li, Liu, He, Bi: "Nitric oxide increases mitochondrial respiration in a cGMP-dependent manner in the callus from Arabidopsis thaliana." dans: Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society, (2010) (PubMed).
Antigène
AOX1/AOX2
(Alternative Oxidase 1/2 (AOX1/AOX2))
Autre désignation
Plant alternative oxidase 1 and 2 (AOX1/2)
Synonymes
anticorps aox2, anticorps ALTERNATIVE OXIDASE, anticorps MSJ1.5, anticorps MSJ1_5, anticorps alternative oxidase 2, anticorps F27G20.12, anticorps alternative oxidase 1D, anticorps Afu2g05060, anticorps alternative oxidase 1, anticorps ubiquinol oxidase 1, mitochondrial, anticorps alternative oxidase 2, anticorps alternative oxidase 1D, anticorps alternative oxidase AlxA, anticorps alternative oxidase, anticorps aox1, anticorps AOX1, anticorps AOX2, anticorps AOX1D, anticorps CNA01500, anticorps aox, anticorps AFUA_2G05060, anticorps Mmar10_2564, anticorps PTOX1, anticorps Cseg_3042, anticorps VOLCADRAFT_65634
Sujet
AGI Code: At3g22370 Alternative oxidases (AOX) are quinol oxidases located in the inner mitochondrial membrane of plants. They function as terminal oxidases in the alternate electron transport pathway, oxidizing ubiquinone to reduce oxygen to water.
Poids moléculaire
expected: 36-40 kDa, apparent: 36-40 for Arabidopsis thaliana