Isocitrate Dehydrogenase anticorps

N° du produit ABIN3197487

Détail du produit anti-Isocitrate Dehydrogenase anticorps

Antigène: Isocitrate Dehydrogenase (IDH)

Reactivité: Arabidopsis thaliana, Pisum sativum, Potato, Tomato, Zea mays

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Isocitrate Dehydrogenase est non-conjugé

Application: Western Blotting (WB)

Réactivité croisée (Details): No cross-reactivity with: Chlamydomonas reinhardtii

Homologie: dicots including Brassica napus, Vitis vinifera, monocots including Oryza sativa, Zea mays

Attributs du produit: Expected / apparent Molecular Weight of the Antigene: 39 / 45 kDa (Arabidopsis thaliana)

Purification: serum

Immunogène: KLH-conjugated peptide 1 and peptide 2 conserved in all higher plants mitochondrial, NAD dependent isocitrate dehydrogenase subunits including Arabidopsis thaliana IDH-I Q8LFC0, At4g35260 and IDH-II P93032, At4g17130

Information d'application

Indications d'application: Recommended Dilution 1 : 5 000 with standard ECL (WB). Additional Information: Peptide used to elicit this antibody is not conserved in NADPH dependentanzymes, partially conserved across eukaryotic Idh subunits. Some conservationacross bacterial which contain the NAD-dependent form of Idh (as opposed to theNADP-dependent form).

Commentaires:

cellular [compartment marker] of mitochondrial matrix

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS pH 7.4

Conseil sur la manipulation: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Stock: -20 °C

Références pour anticorps anti-Isocitrate Dehydrogenase (ABIN3197487)

Rurek, Woyda-Ploszczyca, Jarmuszkiewicz et al.: "Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." dans: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).

Lee, Lee, Yoo, Duncan, Oh, Lee, Lee, Whelan, Hwang: "Mitochondrial targeting of the Arabidopsis F1-ATPase γ-subunit via multiple compensatory and synergistic presequence motifs." dans: The Plant cell, Vol. 24, Issue 12, pp. 5037-57, (2013) (PubMed).

Szal, Jastrz?bska, Kulka, Lea: "Influence of mitochondrial genome rearrangement on cucumber leaf carbon and nitrogen metabolism." dans: Planta, Vol. 232, Issue 6, pp. 1371-82, (2010) (PubMed).

Détails sur Isocitrate Dehydrogenase

Antigène: Isocitrate Dehydrogenase (IDH)

Abstract: IDH Produits

Synonymes: anticorps CG7176, anticorps CT22171, anticorps Dmel\\CG7176, anticorps IDH, anticorps IDH-NADP, anticorps Idh-NADP, anticorps idh, anticorps isocitrate dehydrogenase, anticorps l(3)L3852, anticorps F20D21.16, anticorps F20D21_16, anticorps SMU.672, anticorps icd, anticorps Isocitrate dehydrogenase, anticorps isocitrate dehydrogenase, anticorps isocitrate dehydrogenase, NADP-dependent, anticorps Monomeric isocitrate dehydrogenase, anticorps ankyrin repeat domain 26-like, anticorps Idh, anticorps ICDH, anticorps idh, anticorps icd, anticorps SSO_RS10660, anticorps JK_RS08640, anticorps LOC618625

Sujet: AGI Code: At4g35260
Plant NADH dependent isocitrate dehydrogenase enzyme is located in mitochondrial matrix. This enzyme is classified as an oxidoreductase and its function is to catalyze a reaction in the citric acid cycle, specifically the sequential dehydrogenation and decarboxylation of isocitrate to form a-ketoglutarate. It removes hydrogens from its substrate, isocitrate. In addition to this process, it functions as a decarboxylase, removing a CO2 from the six-carbon substrate to form a five-carbon product mentioned above as a-ketoglutarate. There are two forms of this enzyme NADP+ and NAD+ dependent.

Poids moléculaire: expected: 39 kDa, apparent: 45 kDa (Arabidopsis thaliana)

UniProt: P93032