This antibody recognises Human VCAM-1, a 110 kDa molecule whose ligand is VLA-4. The antigen is expressed on activated endothelial cells and some tissue macrophages, bone marrow fibroblasts and myoblasts.
Flow Cytometry: use 10 μL of neat-1/10 diluted antibody to label 10^6 cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.1 mg/mL
Buffer
PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 0.1 % BSA as stabilizer.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
VCAM1 is important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the integrins alpha4 beta1 (beta 1 integrin VLA4) and alpha4 beta7 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. VCAM1 is also expressed by several non endothelial cell types including some macrophages, follicular dendritic cells and bone marrow, stromal cells.Synonyms: INCAM-100, L1CAM, V-CAM 1, VCAM-1, Vascular cell adhesion protein 1