1. Western blotting: ~1 g/mL 2. Immunofluorescence staining 3. Flow Cytometry: 1 g for 10^6 cells
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
PBS, 50 % glycerol
Agent conservateur
Azide free
Stock
-20 °C
Lopez-Bigas, Kisiel, Dewaal, Holmes, Volkert, Gupta, Love, Murray, Young, Benevolenskaya: "Genome-wide analysis of the H3K4 histone demethylase RBP2 reveals a transcriptional program controlling differentiation." dans: Molecular cell, Vol. 31, Issue 4, pp. 520-30, (2008) (PubMed).
Christensen, Agger, Cloos, Pasini, Rose, Sennels, Rappsilber, Hansen, Salcini, Helin: "RBP2 belongs to a family of demethylases, specific for tri-and dimethylated lysine 4 on histone 3." dans: Cell, Vol. 128, Issue 6, pp. 1063-76, (2007) (PubMed).
Klose, Yan, Tothova, Yamane, Erdjument-Bromage, Tempst, Gilliland, Zhang, Kaelin: "The retinoblastoma binding protein RBP2 is an H3K4 demethylase." dans: Cell, Vol. 128, Issue 5, pp. 889-900, (2007) (PubMed).
RBP2 was originally identified as a retinoblastoma binding protein. It is also known as JARID1A (Jumonji, AT rich interactive domain 1A). RBP2 plays both negative and positive roles in RB- mediated transcriptional activation, depending on the kinds of genes and regulates differentiation by its function as an H3K4 histone demethylase.