TNF alpha anticorps (AA 78-234)
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- Antigène Voir toutes TNF alpha Anticorps
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
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Épitope
- AA 78-234
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Reactivité
- Mouton
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp TNF alpha est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Fonction
- Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)
- Séquence
- MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LRS SSQASNNKPV AHVVANISAP GQLRWGDSYA NALMANGVEL KDNQLVVPTD GLYLIYSQVL FRGHGCPSTP LFLTHTISRI AVSYQTKVNI LSAIKSPCHR ETLEGAEAKP WYEPIYQGGV FQLEKGDRLS AEINLPEYLD YAESGQVYFG IIAL
- Specificité
- The antibody is a mouse monoclonal antibody raised against TNFa. It has been selected for its ability to recognize TNFa in immunohistochemical staining and western blotting.
- Purification
- Protein A + Protein G affinity chromatography
- Immunogène
- Recombinant Tumor Necrosis Factor Alpha (TNFa) corresdonding to Leu78~Leu234 with N-terminal His Tag
- Clone
- 8-3#
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product TNF alpha Anticorps primaire
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- Indications d'application
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Western blotting: 0.5-2 μg/mL
Immunohistochemistry: 5-20 μg/mL
Immunocytochemistry: 5-20 μg/mL
Optimal working dilutions must be determined by end user.
- Commentaires
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Agent conservateur
- ProClin
- Précaution d'utilisation
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Conseil sur la manipulation
- Avoid repeated freeze/thaw cycles
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Date de péremption
- 24 months
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- Antigène
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
- Autre désignation
- Tumor Necrosis Factor Alpha (TNF alpha Produits)
- Synonymes
- anticorps DIF, anticorps TNF-alpha, anticorps TNFA, anticorps TNFSF2, anticorps RATTNF, anticorps Tnfa, anticorps tnf, anticorps TNF-a, anticorps TNFalpha, anticorps Tnfsf1a, anticorps TNFa, anticorps cTNF, anticorps Tnf-alpha, anticorps tnfa-like, anticorps TNF-ALPHA, anticorps dif, anticorps tnfa, anticorps xtnf, anticorps tnfsf2, anticorps tnf-alpha, anticorps Cachectin, anticorps tumor necrosis factor, anticorps tumor necrosis factor b (TNF superfamily, member 2), anticorps tumor necrosis factor alpha, anticorps tumor necrosis factor a (TNF superfamily, member 2), anticorps TNF, anticorps Tnf, anticorps tnf, anticorps tnfb, anticorps tnf-alpha, anticorps LOC103694380, anticorps tnfa
- Sujet
- DIF, TNF-A, TNFSF2, Cachectin, Tumor Necrosis Factor Ligand Superfamily Member 2
- Pathways
- Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
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