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PTH1R anticorps (AA 146-169)

PTH1R Reactivité: Humain, Souris, Boeuf (Vache), Porc, Chien, Singe, Cheval WB, IHC, IHC (p) Hôte: Souris Monoclonal unconjugated
N° du produit ABIN334275
  • Antigène Voir toutes PTH1R Anticorps
    PTH1R (Parathyroid Hormone 1 Receptor (PTH1R))
    Épitope
    • 6
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 146-169
    Reactivité
    • 42
    • 17
    • 11
    • 7
    • 7
    • 5
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain, Souris, Boeuf (Vache), Porc, Chien, Singe, Cheval
    Hôte
    • 35
    • 6
    • 1
    Souris
    Clonalité
    • 36
    • 6
    Monoclonal
    Conjugué
    • 31
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp PTH1R est non-conjugé
    Application
    • 28
    • 18
    • 16
    • 9
    • 8
    • 7
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marque
    IHC-plus™
    Specificité
    Recognizes human PTH/PTHrP receptor, MW 80kD, and the receptor dimer, Mr 160kD. A faint nonspecific band at 110kD is also detected. This antibody does not detect the PTH2 receptor.
    Homologie
    Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Bovine, Dog, Horse, Pig (100%) Mouse, Rat, Hamster, Panda, Bat, Rabbit (96%) Opossum, Chicken (92%) Xenopus (88%).
    Purification
    Protein G purified
    Immunogène
    Synthetic peptide corresponding to aa146-169 of human PTH/PTHrP Receptor [RRCDRNGSWELVPGHNRTWANYSE]. Species sequence Homology: Mouse & rat (23/24aa). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Bovine, Dog, Horse, Pig (100%), Mouse, Rat, Hamster, Panda, Bat, Rabbit (96%), Opossum, Chicken (92%), Xenopus (88%).

    Type of Immunogen: Synthetic peptide
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product PTH1R Anticorps primaire
  • Indications d'application
    Approved: IHC, IHC-P (5 μg/mL), WB (0.5 - 2 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Detects PTH/PTHrP receptor in human kidney, vascular smooth muscle and ovary sections. Receptor was also detected in porcine oocytes. Western Blot: 0.5-2 μg/mL. Detects PTH/PTHrP receptor from RIPA lysates of 3T3-L1 pre-adipose fibroblasts. 3T3-L1 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with P3109-50. Proteins were visualized using an IgG (HRP) goat anti-mouse secondary antibody and a chemiluminescence detection system.
    Commentaires

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS,  pH 7.6, 0.07 % sodium azide, 30 % glycerol
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeat freeze-thaw cycles.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • Antigène
    PTH1R (Parathyroid Hormone 1 Receptor (PTH1R))
    Autre désignation
    PTHR / PTHR1 (PTH1R Produits)
    Synonymes
    anticorps PFE, anticorps PTHR, anticorps PTHR1, anticorps PPR, anticorps Pthr, anticorps Pthr1, anticorps pthr1, anticorps zPTH1R, anticorps PTHrel, anticorps PTH1, anticorps PTH, anticorps parathyroid hormone 1 receptor, anticorps parathyroid hormone 1 receptor a, anticorps PTH1R, anticorps Pth1r, anticorps pth1ra
    Sujet
    Name/Gene ID: PTH1R
    Subfamily: Parathyroid hormone
    Family: GPCR

    Synonyms: PTH1R, Parathyroid hormone 1 receptor, Parathyroid hormone receptor 1, PFE, Pth-related peptide receptor, PTHR, PTH/PTHr receptor, Pth/pthrp receptor, Parathyroid hormone receptor, PTH/PTHrP type I receptor, PTH1 receptor, PTHR1, PTHrP-R, PTHrP-receptor
    ID gène
    5745
    UniProt
    Q03431
    Pathways
    Regulation of Carbohydrate Metabolic Process
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