GAD anticorps (Internal Region)

N° du produit ABIN334405

Détail du produit anti-GAD anticorps

Antigène: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Épitope: Internal Region

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp GAD est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Fonction: GAD1 (isoform GAD67)

Séquence: PDSPQRREKL HK

Specificité: This antibody is expected to recognize isoform GAD67. There is no cross-reactivity expected with GAD2.

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité: Verified

Immunogène: Peptide with sequence C-PDSPQRREKLHK, from the internal region of the protein sequence according to NP_000808.2.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 65-70 kDa band observed in Human Cerebral Cortex lysates (calculated MW of 66.9 kDa according to NP_000808.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-GAD (ABIN334405)

Magri, Giacopuzzi, La Via, Bonini, Ravasio, Elhussiny, Orizio, Gangemi, Valsecchi, Bresciani, Barbon, Vita, Gennarelli: "A novel homozygous mutation in GAD1 gene described in a schizophrenic patient impairs activity and dimerization of GAD67 enzyme." dans: Scientific reports, Vol. 8, Issue 1, pp. 15470, (2018) (PubMed).

Détails sur GAD

Antigène: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Autre désignation: GAD1 (GAD1 Produits)

Sujet: GAD1, glutamate decarboxylase 1 (brain, 67 kDa), FLJ45882, GAD, SCP, OTTHUMP00000041055, glutamate decarboxylase 1, glutamate decarboxylase 1 (brain, 67kD), GAD67, GAD25

ID gène: 2571, 14415, 24379

NCBI Accession: NP_000808