ITCH anticorps (Internal Region)

N° du produit ABIN334426

Détail du produit anti-ITCH anticorps

Antigène: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Épitope: Internal Region

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp ITCH est non-conjugé

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Fonction: ITCH / AIF4

Séquence: EIKSHDLKPN GGN

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité: Verified

Immunogène: Peptide with sequence C-EIKSHDLKPNGGN, from the internal region of the protein sequence according to NP_113671.3.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 90 kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 86.4 kDa according to NP_001244067.1) . Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:16000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur ITCH

Antigène: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Autre désignation: ITCH (ITCH Produits)

Synonymes: anticorps AIF4, anticorps AIP4, anticorps NAPP1, anticorps dJ468O1.1, anticorps 6720481N21Rik, anticorps 8030492O04Rik, anticorps A130065M08, anticorps C230047C07Rik, anticorps itchy E3 ubiquitin protein ligase, anticorps itchy, E3 ubiquitin protein ligase, anticorps ITCH, anticorps Itch

Sujet: ITCH, AIF4, itchy homolog E3 ubiquitin protein ligase (mouse) , AIP4, NAPP1, dJ468O1.1 , NFE2-associated polypeptide 1, atrophin-1 interacting protein 4, dJ468O1.1 (atrophin 1 interacting protein 4, AIP4), itchy homolog E3 ubiquitin protein ligase, ubiqui

ID gène: 83737, 16396, 311567

NCBI Accession: NP_113671, NP_001244066, NP_001244067

Pathways: Activation of Innate immune Response, CXCR4-mediated Signaling Events