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MBD2 anticorps (Internal Region)

Verified MBD2 Reactivité: Humain WB, ELISA, IHC Hôte: Chèvre Polyclonal unconjugated
N° du produit ABIN334432
  • Antigène Voir toutes MBD2 Anticorps
    MBD2 (Methyl-CpG Binding Domain Protein 2 (MBD2))
    Épitope
    • 9
    • 8
    • 7
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivité
    • 47
    • 23
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Humain
    Hôte
    • 45
    • 2
    Chèvre
    Clonalité
    • 44
    • 3
    Polyclonal
    Conjugué
    • 25
    • 4
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp MBD2 est non-conjugé
    Application
    • 40
    • 21
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Fonction
    MBD2 (isoform 1)
    Séquence
    RNDPLNQNKG KPDLN
    Specificité
    This antibody is expected to resognize isoform 1 (NP_003918.1) only.
     Réactivité croisée
    Chien, Humain, Souris, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Classe de qualité
    Verified
    Immunogène
    Peptide with sequence C-RNDPLNQNKGKPDLN, from the internal region of the protein sequence according to NP_003918.1.
    Isotype
    IgG
  • Indications d'application

    Immunohistochemistry: Paraffin embedded Human Colon, Adrenal Gland and Skin. Recommended concentration: 2.5 μg/mL.

    Western Blot: Approx 48-50 kDa band observed in lysates of cell line Jurkat (calculated MW of 43.3 kDa according to NP_003918.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Tan et al, Mol Cell Bio

    Peptide ELISA: antibody detection limit dilution 1:64000.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Minimize freezing and thawing.
    Stock
    -20 °C
    Stockage commentaire
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Bartels, Spruijt, Brinkman, Jansen, Vermeulen, Stunnenberg: "A SILAC-based screen for Methyl-CpG binding proteins identifies RBP-J as a DNA methylation and sequence-specific binding protein." dans: PLoS ONE, Vol. 6, Issue 10, pp. e25884, (2012) (PubMed).

    Spruijt, Bartels, Brinkman, Tjeertes, Poser, Stunnenberg, Vermeulen: "CDK2AP1/DOC-1 is a bona fide subunit of the Mi-2/NuRD complex." dans: Molecular bioSystems, Vol. 6, Issue 9, pp. 1700-6, (2010) (PubMed).

  • Antigène
    MBD2 (Methyl-CpG Binding Domain Protein 2 (MBD2))
    Autre désignation
    MBD2 (MBD2 Produits)
    Synonymes
    anticorps MBD2, anticorps DMTase, anticorps NY-CO-41, anticorps MBD2a, anticorps wu:fa97f06, anticorps zgc:111862, anticorps methyl-CpG binding domain protein 2, anticorps methyl-CpG binding domain protein 2 S homeolog, anticorps MBD2, anticorps Mbd2, anticorps mbd2, anticorps mbd2.S
    Sujet
    MBD2, methyl-CpG binding domain protein 2 , DKFZp586O0821, DMTase, NY-CO-41
    ID gène
    8932, 17191
    NCBI Accession
    NP_003918
    Pathways
    Chromatin Binding
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