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TOP2 anticorps (C-Term, N-Term)

TOP2 Reactivité: Arabidopsis thaliana, Vicia faba WB, IL Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN334538
  • Antigène Voir toutes TOP2 Anticorps
    TOP2 (DNA Topoisomerase II (TOP2))
    Épitope
    • 8
    • 6
    • 1
    • 1
    • 1
    • 1
    C-Term, N-Term
    Reactivité
    • 12
    • 2
    • 1
    Arabidopsis thaliana, Vicia faba
    Hôte
    • 11
    • 4
    Lapin
    Clonalité
    • 10
    • 5
    Polyclonal
    Conjugué
    • 10
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp TOP2 est non-conjugé
    Application
    • 15
    • 7
    • 7
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunolocalization (IL)
    Specificité
    Antibody detects a protein of ca. 170 kDa on western blots of Arabidopsis thaliana protein extracts. In subcellular fractions of cultured Arabidopsis cells the antibody detects a 170 kDa protein exclusively in the nulear fraction.
    Réactivité croisée (Details)
    Not reactive in: Nicotiana tabacum
    Homologie
    Brassica rapa, Chlamydomonas reinhardtii, Chlorella vulgaris, Citrus clementina, Glycine max, Hordeum vulgare, Medicago truncatula, Oryza sativa, Ostreococcus tauri, Panicum italicum, Phaseolus vulgaris, Physcomitrella patens, Pinus sitchensis, Populus trichocarpa, Solanum tuberosum, Sorghum bicolor, Triticum aestivum, Vitis vinifera, Volvox caterii
    Attributs du produit
    Expected / apparent Molecular Weight of the Antigene: 164 / 170 kDa (Arabidopsis thaliana)
    Purification
    serum
    Immunogène
    The C-terminal 153 amino acids of the Arabidopsis thaliana Topoisomerase II (At3g23890, protein accesion number P30182) with an N-terminal hexahistidine tag was expressed in E.coli and purified by Ni2+ affinity chromatography.
    Top Product
    Discover our top product TOP2 Anticorps primaire
  • Indications d'application
    1: 2000 (WB), 1: 500 (IL)
    Commentaires

    Topoisomerase II is highly expressed in young seedlings. The protein is localized in the nucleus and gene expression levels are increased in proliferative tissues like shoot apex or root tip.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    For reconstitution add 200 μL of sterile water
    Conseil sur la manipulation
    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
    Stock
    -20 °C
    Stockage commentaire
    store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Xie, Lam: "Characterization of a DNA Topoisomerase II cDNA from Arabidopsis thaliana." dans: Plant physiology, Vol. 106, Issue 4, pp. 1701-2, (1995) (PubMed).

  • Antigène
    TOP2 (DNA Topoisomerase II (TOP2))
    Autre désignation
    TOP2 (TOP2 Produits)
    Synonymes
    anticorps Topoii, anticorps ptr11, anticorps DDBDRAFT_0191045, anticorps DDBDRAFT_0231510, anticorps DDB_0191045, anticorps DDB_0231510, anticorps CG10223, anticorps CHRAC, anticorps Dmel\\CG10223, anticorps TOP2, anticorps TOPO II, anticorps TOPOII, anticorps TopII, anticorps Topo II, anticorps TopoII, anticorps dCHRAC, anticorps dTopoII, anticorps top2, anticorps topo II, anticorps topoII, anticorps topoisomerase II, anticorps DNA topoisomerase II, anticorps DNA topoisomerase 2, anticorps DNA topoisomerase ii, anticorps type IIA topoisomerase; DNA gyrase topoisomerase IV B subunit, anticorps Topoisomerase 2, anticorps TOP2, anticorps A583L, anticorps top2, anticorps CNA03600, anticorps Tb09.160.4090, anticorps Tb11.01.3390, anticorps LMJF_28_2280, anticorps Top2
    Sujet
    AGI Code: At3g23890
    Topoisomerase type II (EC5.99.1.3) is one of the enzymes which is catalyzing unknotting of DNA by creating transient breaks in the DNA using a conserved tyrosine as the catalytic residue.Synonyme names of this protein: At3g23890, ATTOPII, DNA topoisomerase 2, DNA topoisomerase II, F14O13.7, TOP2, TOPOISOMERASE II
    Poids moléculaire
    expected: 164 kDa, apparent: 170 kDa (Arabidopsis thaliana)
    UniProt
    P30182
    Pathways
    Mitotic G1-G1/S Phases
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