Collagen IV anticorps

N° du produit ABIN335193

Cité dans 4 publications. Cet anticorps anti-Collagen IV Polyclonal Lapin (ABIN335193) détecte spécifiquement Collagen IV dans WB, IHC, IHC (fro), IHC (p) et ICC. L’anticorps est réactif avec des échantillons de Humain, Rat, Porc et Boeuf (Vache).

Aperçu rapide pour Collagen IV anticorps (ABIN335193)

Antigène: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reactivité: Humain, Rat, Porc, Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Collagen IV est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Détail du produit anti-Collagen IV anticorps

Specificité: Human, rat, pig, cow.

Purification: Antiserum

Immunogène: This polyclonal type IV collagen antibody is a rabbit polyclonal antibody. The rabbits were immunized with isolated human type IV collagen. The immunogen has been isolated from human placenta.

Information d'application

Indications d'application: Polyclonal type IV collagen recognizes the extracellular - basement membranes. Polyclonal type IV is useful for immunoblotting, immunocytochemistry on fixed cells (methanol fixation) and immunohistochemistry on frozen tissues when using a PBS buffer containing 0.1 mM CaCl 2 and 0.1 mM MgCl 2. For immunohistochemistry on paraffin sections the sections should be pretreated with 1 mg/ml pepsin in 0.01 N HCl for 30 minutes at room temperature. Optimal antibody dilution should be determined by titration, 1:10 - 1:20 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Restrictions: For Research Use only

Stockage

Format: Liquid

Stock: 4 °C

Références pour anticorps anti-Collagen IV (ABIN335193)

Cleutjens, Havenith, Kasper, Vallinga, Bosman: "Absence of type IV collagen in the centre of the corneal epithelial basement membrane." dans: The Histochemical journal, Vol. 22, Issue 12, pp. 688-94, (1991) (PubMed).

Schapers, Pauwels, Havenith, Smeets, van den Brandt, Bosman: "Prognostic significance of type IV collagen and laminin immunoreactivity in urothelial carcinomas of the bladder." dans: Cancer, Vol. 66, Issue 12, pp. 2583-8, (1991) (PubMed).

Cleutjens, Havenith, Beek, Vallinga, Ten Kate, Bosman: "Origin of basement membrane type IV collagen in xenografted human epithelial tumor cell lines." dans: The American journal of pathology, Vol. 136, Issue 5, pp. 1165-72, (1990) (PubMed).

Visser, van der Beek, Havenith, Cleutjens, Bosman: "Immunocytochemical detection of basement membrane antigens in the histopathological evaluation of laryngeal dysplasia and neoplasia." dans: Histopathology, Vol. 10, Issue 2, pp. 171-80, (1986) (PubMed).

Détails sur Collagen IV

Antigène: Collagen IV (COL4) (Collagen, Type IV (COL4))

Autre désignation: Collagen Type IV