KRT14 anticorps

N° du produit ABIN335329

Détail du produit anti-KRT14 anticorps

Antigène: KRT14 (Keratin 14 (KRT14))

Reactivité: Humain, Rat, Porc, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp KRT14 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: Human, rat, canine and swine.

Purification: Purified

Immunogène: RCK107 is a mouse monoclonal IgG3 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a mouse immunized with a cytoskeletal preparation of TR146 epithelial cells.

Clone: RCK107

Isotype: IgG1

Information d'application

Indications d'application: RCK107 reacts exclusively with cytokeratin 14 which is present in basal cell compartments of stratified and combined epithelia. RCK107 is suitable for immunocytochemistry, immunohistochemistry on frozen sections, flow cytometry and immunoblotting. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/ml

Stock: 4 °C

Références pour anticorps anti-KRT14 (ABIN335329)

Meek, Van Elssen, Huijskens, van der Stegen, Tonnaer, Lumeij, Vanderlocht, Hesselink, Kirkland, Germeraad, Bos: "T cells fail to develop in the human skin-cell explants system, an inconvenient truth." dans: BMC immunology, Vol. 12, Issue 1, pp. 17, (2011) (PubMed).

van Leenders, Dijkman, Hulsbergen-van de Kaa, Ruiter, Schalken: "Demonstration of intermediate cells during human prostate epithelial differentiation in situ and in vitro using triple-staining confocal scanning microscopy." dans: Laboratory investigation; a journal of technical methods and pathology, Vol. 80, Issue 8, pp. 1251-8, (2000) (PubMed).

Vos, van den Ingh, de Neijs, van Mil, Ivanyi, Ramaekers: "Immunohistochemistry with keratin monoclonal antibodies in canine tissues: urogenital tract, respiratory tract, (neuro-)endocrine tissues, choroid plexus and spinal cord." dans: Zentralblatt für Veterinärmedizin. Reihe A, Vol. 39, Issue 10, pp. 721-40, (1993) (PubMed).

Vos, van den Ingh, Ramaekers, Molenbeek, de Neijs, van Mil, Ivanyi: "The expression of keratins, vimentin, neurofilament proteins, smooth muscle actin, neuron-specific enolase, and synaptophysin in tumors of the specific glands in the canine anal region." dans: Veterinary pathology, Vol. 30, Issue 4, pp. 352-61, (1993) (PubMed).

Bauwens, De Groot, Ramaekers, Veldman, Huizing: "Expression of intermediate filament proteins in the adult human vestibular labyrinth." dans: The Annals of otology, rhinology, and laryngology, Vol. 101, Issue 6, pp. 479-86, (1992) (PubMed).

Smedts, Ramaekers, Troyanovsky, Pruszczynski, Link, Lane, Leigh, Schijf, Vooijs: "Keratin expression in cervical cancer." dans: The American journal of pathology, Vol. 141, Issue 2, pp. 497-511, (1992) (PubMed).

Smedts, Ramaekers, Troyanovsky, Pruszczynski, Robben, Lane, Leigh, Plantema, Vooijs: "Basal-cell keratins in cervical reserve cells and a comparison to their expression in cervical intraepithelial neoplasia." dans: The American journal of pathology, Vol. 140, Issue 3, pp. 601-12, (1992) (PubMed).

Wetzels, Schaafsma, Leigh, Lane, Troyanovsky, Wagenaar, Vooijs, Ramaekers: "Laminin and type VII collagen distribution in different types of human lung carcinoma: correlation with expression of keratins 14, 16, 17 and 18." dans: Histopathology, Vol. 20, Issue 4, pp. 295-303, (1992) (PubMed).

Wetzels, Kuijpers, Lane, Leigh, Troyanovsky, Holland, van Haelst, Ramaekers: "Basal cell-specific and hyperproliferation-related keratins in human breast cancer." dans: The American journal of pathology, Vol. 138, Issue 3, pp. 751-63, (1991) (PubMed).

Détails sur KRT14

Antigène: KRT14 (Keratin 14 (KRT14))

Autre désignation: Cytokeratin 14 / Keratin K14 (KRT14 Produits)

Synonymes: anticorps CK14, anticorps EBS3, anticorps EBS4, anticorps K14, anticorps NFJ, anticorps AI626930, anticorps Krt-1.14, anticorps Krt1-14, anticorps Ka14, anticorps ouro2, anticorps keratin 14, anticorps keratin 14, type I L homeolog, anticorps KRT14, anticorps Krt14, anticorps krt14.L

Sujet: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.