Desmin anticorps

N° du produit ABIN335360

Détail du produit anti-Desmin anticorps

Antigène: Desmin (DES)

Reactivité: Humain, Souris, Rat, Poulet, Hamster, Porc, Chien, Lapin, Poisson zèbre (Danio rerio)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Desmin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: Human, mouse, rat, rabbit, hamster, canine, chicken and swine.

Purification: Purified

Immunogène: RD301 is a mouse monoclonal IgG2b antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a mouse immunized with a cytoskeletal desmin extract of chicken gizzard.

Clone: RD301

Isotype: IgG2b

Information d'application

Indications d'application: RD301 reacts exclusively with desmin, which is expressed in smooth and striated muscle cells and their tumors e.g. rhabdomyosarcoma and leiomyosarcoma. RD301 is suitable for immunoblotting and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Références pour anticorps anti-Desmin (ABIN335360)

Council, Hameed: "Differential expression of immunohistochemical markers in bladder smooth muscle and myofibroblasts, and the potential utility of desmin, smoothelin, and vimentin in staging of bladder carcinoma." dans: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 22, Issue 5, pp. 639-50, (2009) (PubMed).

Dispersyn, Geuens, Ver Donck, Ramaekers, Borgers: "Adult rabbit cardiomyocytes undergo hibernation-like dedifferentiation when co-cultured with cardiac fibroblasts." dans: Cardiovascular research, Vol. 51, Issue 2, pp. 230-40, (2001) (PubMed).

Schaart, Pieper, Kuijpers, Bloemendal, Ramaekers: "Baby hamster kidney (BHK-21/C13) cells can express striated muscle type proteins." dans: Differentiation; research in biological diversity, Vol. 46, Issue 2, pp. 105-15, (1991) (PubMed).

Raats, Pieper, Vree Egberts, Verrijp, Ramaekers, Bloemendal: "Assembly of amino-terminally deleted desmin in vimentin-free cells." dans: The Journal of cell biology, Vol. 111, Issue 5 Pt 1, pp. 1971-85, (1990) (PubMed).

Schaart, Viebahn, Langmann, Ramaekers: "Desmin and titin expression in early postimplantation mouse embryos." dans: Development (Cambridge, England), Vol. 107, Issue 3, pp. 585-96, (1990) (PubMed).

Pieper, Schaart, Krimpenfort, Henderik, Moshage, van de Kemp, Ramaekers, Berns, Bloemendal: "Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells." dans: The Journal of cell biology, Vol. 108, Issue 3, pp. 1009-24, (1989) (PubMed).

Verhagen, Aalders, Ramaekers, Debruyne, Schalken: "Differential expression of keratins in the basal and luminal compartments of rat prostatic epithelium during degeneration and regeneration." dans: The Prostate, Vol. 13, Issue 1, pp. 25-38, (1988) (PubMed).

Quax, van den Broek, Egberts, Ramaekers, Bloemendal: "Characterization of the hamster desmin gene: expression and formation of desmin filaments in nonmuscle cells after gene transfer." dans: Cell, Vol. 43, Issue 1, pp. 327-38, (1986) (PubMed).

Détails sur Desmin

Antigène: Desmin (DES)

Autre désignation: Desmin (DES Produits)

Synonymes: anticorps DES, anticorps des-b, anticorps MGC80853, anticorps des, anticorps csm1, anticorps csm2, anticorps desm, anticorps cmd1i, anticorps MGC75911, anticorps LOC100220724, anticorps desmin, anticorps cb290, anticorps fb59a12, anticorps wu:fb59a12, anticorps zgc:109859, anticorps CSM1, anticorps CSM2, anticorps LGMD2R, anticorps wu:fc11d08, anticorps zgc:154009, anticorps des-a, anticorps MGC52614, anticorps desmin, anticorps desmin, gene 1 L homeolog, anticorps desmin, gene 1, anticorps desmin a, anticorps desmin b, anticorps desmin, gene 1 S homeolog, anticorps desmin, gene 2 S homeolog, anticorps DES, anticorps des.1.L, anticorps des.1, anticorps des, anticorps desma, anticorps Des, anticorps desmb, anticorps des.1.S, anticorps des.2.S

Sujet: Desmin is a 53 kDa intermediate filament protein that exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development of, for example, skeletal muscle cell differentiation. In skeletal en cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of desmin in smooth muscle is much more heterogenous. Coexpression of vimentin and desmin has been observed in tumors derived from muscle tissue, i.e. rhabdomyosarcomas and leiomyosarcomas. Furthermore, during myocard dysfunction dramatic changes in the distribution of desmin have been observed.