Lamin B2 anticorps

N° du produit ABIN335392

Cet anticorps Souris Monoclonal détecte spécifiquement Lamin B2 dans WB, IHC et FACS. Il présente une réactivité avec des échantillons de Humain, Souris, Xenopus laevis et Hamster. Il a été cité dans 8+ publications.

Aperçu rapide pour Lamin B2 anticorps (ABIN335392)

Antigène: Lamin B2 (LMNB2)

Reactivité: Humain, Souris, Xenopus laevis, Hamster

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Lamin B2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: LN43

Détail du produit anti-Lamin B2 anticorps

Specificité: Human, mouse, hamster and Xenopus.

Purification: Purified

Immunogène: LN43 is a mouse monoclonal IgG1 antibody derived by fusion of mouse myeloma cells with spleen cells from a mouse immunized with the detergent insoluble fraction of potoroo cell line PtK1.

Isotype: IgG1

Information d'application

Indications d'application: LN43 reacts with an epitope located in the C-terminal part of lamin B2. LN43 is suitable for immunohistochemistry on frozen sections, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Références pour anticorps anti-Lamin B2 (ABIN335392)

Broers, Machiels, Kuijpers, Smedts, van den Kieboom, Raymond, Ramaekers: "A- and B-type lamins are differentially expressed in normal human tissues." dans: Histochemistry and cell biology, Vol. 107, Issue 6, pp. 505-17, (1997) (PubMed).

Jansen, Machiels, Hopman, Broers, Bot, Arends, Ramaekers, Schouten: "Comparison of A and B-type lamin expression in reactive lymph nodes and nodular sclerosing Hodgkin's disease." dans: Histopathology, Vol. 31, Issue 4, pp. 304-12, (1997) (PubMed).

Machiels, Ramaekers, Kuijpers, Groenewoud, Oosterhuis, Looijenga: "Nuclear lamin expression in normal testis and testicular germ cell tumours of adolescents and adults." dans: The Journal of pathology, Vol. 182, Issue 2, pp. 197-204, (1997) (PubMed).

Machiels, Zorenc, Endert, Kuijpers, van Eys, Ramaekers, Broers: "An alternative splicing product of the lamin A/C gene lacks exon 10." dans: The Journal of biological chemistry, Vol. 271, Issue 16, pp. 9249-53, (1996) (PubMed).

Machiels, Broers, Raymond, de Ley, Kuijpers, Caberg, Ramaekers: "Abnormal A-type lamin organization in a human lung carcinoma cell line." dans: European journal of cell biology, Vol. 67, Issue 4, pp. 328-35, (1996) (PubMed).

Hozák, Sasseville, Raymond, Cook: "Lamin proteins form an internal nucleoskeleton as well as a peripheral lamina in human cells." dans: Journal of cell science, Vol. 108 ( Pt 2), pp. 635-44, (1995) (PubMed).

Jenkins, Hölman, Lyon, Lane, Stick, Hutchison: "Nuclei that lack a lamina accumulate karyophilic proteins and assemble a nuclear matrix." dans: Journal of cell science, Vol. 106 ( Pt 1), pp. 275-85, (1994) (PubMed).

Bridger, Kill, OFarrell, Hutchison: "Internal lamin structures within G1 nuclei of human dermal fibroblasts." dans: Journal of cell science, Vol. 104 ( Pt 2), pp. 297-306, (1993) (PubMed).

Détails sur Lamin B2

Antigène: Lamin B2 (LMNB2)

Autre désignation: Lamin B2

Sujet: Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, ie lamin B1 and lamin B2.

Pathways: Apoptose, Caspase Cascade in Apoptosis